Bonchev Georgi, Dusinský Roman, Hauptvogel Pavol, Švec Miroslav
Laboratory of Genome Dynamics and Stability, Institute of Plant Physiology and Genetics, Acad. G. Bonchev Str, Build. 21, 1113, Sofia, Bulgaria.
Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, 842 15, Bratislava 4, Slovakia.
J Mol Evol. 2017 Mar;84(2-3):116-128. doi: 10.1007/s00239-016-9779-z. Epub 2017 Feb 6.
The patterns of genetic diversity related to the taxonomy and domestication history of 85 accessions representing the main four species of the genus Hordeum were examined by retrotransposon-microsatellite amplified polymorphism (REMAP) markers based on the retrotransposon BARE-1. A substantial level of genetic polymorphisms at among- and within-species level was observed showing that this retrotransposon family and its adjacent genomic regions has been a target for genome dynamics during the evolution and domestication of barley. The obtained data are consistent with the current taxonomic status within the genus Hordeum. Similar level of genetic diversity was observed between the wild and the domesticated barley accessions suggesting that transposable elements` activity and accumulation may counteract the decrease of genome-wide diversity following domestication. In addition, eco-geographical sub-genome pools of the cultivated barley were identified in support to the theory of multiple origins of domestication within the genus Hordeum. We also provide conclusions about the relationship between accessions of different species and the putative routes of barley domestication. In conclusion, the retrotransposon BARE-1 stands as a reliable and perspective DNA marker for the assessment of the phylogenetic and domestication history in the genus Hordeum and other crop species.
基于反转录转座子BARE-1，利用反转录转座子-微卫星扩增多态性（REMAP）标记，研究了代表大麦属四个主要物种的85份材料的遗传多样性模式与分类学及驯化历史的关系。在种间和种内水平均观察到了相当程度的遗传多态性，表明该反转录转座子家族及其邻近的基因组区域在大麦的进化和驯化过程中一直是基因组动态变化的靶点。所得数据与大麦属目前的分类地位一致。野生和驯化大麦材料之间观察到相似水平的遗传多样性，这表明转座元件的活性和积累可能抵消了驯化后全基因组多样性的降低。此外，确定了栽培大麦的生态地理亚基因组库，以支持大麦属内多起源驯化的理论。我们还给出了不同物种材料之间的关系以及大麦驯化可能途径的结论。总之，反转录转座子BARE-1是评估大麦属及其他作物物种系统发育和驯化历史的可靠且有前景的DNA标记。
