Department of Neurosurgery, School of Medicine, Emory University, Atlanta, Georgia, USA.
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Stem Cells Transl Med. 2017 Jan;6(1):139-150. doi: 10.5966/sctm.2015-0422. Epub 2016 Aug 29.
We report on the diagnostic capability of magnetic resonance imaging (MRI)-based tracking of ferumoxytol-labeled human neural progenitor cells (hNPCs) transplanted into the porcine spinal cord. hNPCs prelabeled with two doses of ferumoxytol nanoparticles (hNPC-F and hNPC-F ) were injected into the ventral horn of the spinal cord in healthy minipigs. Ferumoxytol-labeled grafts were tracked in vivo up to 105 days after transplantation with MRI. Injection accuracy was assessed in vivo at day 14 and was predictive of "on" or "off" target cell graft location assessed by histology. No difference in long-term cell survival, assessed by quantitative stereology, was observed among hNPC-F , hNPC-F , or control grafts. Histological iron colocalized with MRI signal and engrafted human nuclei. Furthermore, the ferumoxytol-labeled cells retained nanoparticles and function in vivo. This approach represents an important leap forward toward facilitating translation of cell-tracking technologies to clinical trials by providing a method of assessing transplantation accuracy, delivered dose, and potentially cell survival. Stem Cells Translational Medicine 2017;6:139-150.
我们报告了基于磁共振成像（MRI）的铁氧体标记的人神经祖细胞（hNPC）移植到猪脊髓后追踪的诊断能力。将两种剂量的铁氧体纳米颗粒（hNPC-F 和 hNPC-F ）预先标记的 hNPC 注入健康小型猪脊髓腹角。在移植后 105 天内，通过 MRI 在体内追踪铁氧体标记的移植物。在第 14 天，通过体内评估注射准确性，可以预测组织学评估的“靶内”或“靶外”细胞移植物位置。通过定量立体学评估，hNPC-F 、hNPC-F 或对照移植物之间的长期细胞存活率无差异。组织学铁与 MRI 信号和植入的人类核共定位。此外，铁氧体标记的细胞在体内保留了纳米颗粒并保持功能。这种方法通过提供一种评估移植准确性、传递剂量和潜在细胞存活的方法，代表了朝着促进细胞追踪技术向临床试验转化迈出的重要一步。《干细胞转化医学》2017 年；6:139-150.
