Fraser I A, Shaffer P, Tuttle S V, Lessler M A, Ellison E C, Carey L C
Department of Surgery, Ohio State University, Columbus.
Am J Surg. 1989 Nov;158(5):423-7. doi: 10.1016/0002-9610(89)90277-8.
Obstructive jaundice was produced in 11 dogs by common bile duct ligation for 4 weeks, then biliary decompression was performed by bilioenteric anastomosis. Animals were regularly studied over the 2 months after decompression by the following methods: (1) serum biochemistry was monitored; (2) light microscopic changes in serial liver biopsies were graded; (3) the respiratory characteristics of isolated hepatic mitochondria, obtained by open liver biopsy, were studied using an oxygen micro-electrode system; (4) in vivo handling of an hepatobiliary imaging agent (diisopropyl iminodiacetic acid) was studied by dynamic liver scintiscanning. None of these liver assessments were normalized after 7 to 10 days of biliary decompression. Our study suggests that biliary decompression of patients with extrahepatic biliary obstruction requires long periods of time to enable major recovery of the abnormal liver function induced by biliary obstruction.
通过结扎胆总管4周，在11只犬中制造出梗阻性黄疸，然后通过胆肠吻合术进行胆道减压。在减压后的2个月内，通过以下方法定期对动物进行研究：（1）监测血清生化指标；（2）对连续肝脏活检的光镜变化进行分级；（3）使用氧微电极系统研究通过开放式肝脏活检获得的分离肝线粒体的呼吸特性；（4）通过动态肝脏闪烁扫描研究肝胆显像剂（二异丙基亚氨基二乙酸）的体内处理情况。在胆道减压7至10天后，这些肝脏评估指标均未恢复正常。我们的研究表明，肝外胆道梗阻患者的胆道减压需要很长时间才能使由胆道梗阻引起的异常肝功能得到主要恢复。
