一种用于检测皮摩尔水平磷脂酸的酶促测定法。
An enzymatic assay for picomole levels of phosphatidate.
作者信息
Bocckino S B, Wilson P, Exton J H
机构信息
Howard Hughes Medical Institute, Nashville, Tennessee.
出版信息
Anal Biochem. 1989 Jul;180(1):24-7. doi: 10.1016/0003-2697(89)90083-3.
PMID:2817344
Abstract
An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosphatidate in small amounts of biological materials.
摘要
本文描述了一种灵敏、特异且高度可重复的磷脂酸检测方法。磷脂酸与甲胺进行转酰基反应，然后对产生的3-磷酸甘油进行酶促测定。该检测方法在50至1500皮摩尔磷脂酸范围内呈线性，可用于定量少量生物材料中的磷脂酸。
Zotero 插件