Champney W S
Department of Biochemistry, College of Medicine, East Tennessee State University, Johnson City 37614.
Anal Biochem. 1989 Aug 15;181(1):90-5. doi: 10.1016/0003-2697(89)90398-9.
Methods for the reductive methylation of the amino groups of eight different antibiotics using 3HCOH or H14COH are presented. The reductive labeling of an additional seven antibiotics by NaB3H4 is also described. The specific activity of the methyl-labeled drugs was determined by a phosphocellulose paper binding assay. Two quantitative assays for these compounds based on the reactivity of the antibiotic amino groups with fluorescamine and of the aldehyde and ketone groups with 2,4-dinitrophenylhydrazine are also presented. Data on the cellular uptake and ribosome binding of these labeled compounds are also presented.
介绍了使用3HCOH或H14COH对八种不同抗生素的氨基进行还原甲基化的方法。还描述了用NaB3H4对另外七种抗生素进行还原标记的方法。通过磷酸纤维素纸结合试验测定甲基标记药物的比活性。还提出了基于抗生素氨基与荧光胺的反应性以及醛基和酮基与2,4-二硝基苯肼的反应性对这些化合物进行的两种定量测定方法。还给出了这些标记化合物的细胞摄取和核糖体结合数据。
