α-突触核蛋白基因内含子 1 的低甲基化与帕金森病无关。
Hypomethylation of intron1 of α-synuclein gene does not correlate with Parkinson's disease.
机构信息
Division of Neurosciences, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd, Orlando, FL, 32827, USA.
Kyunghee University Medical College, Seoul, Korea.
出版信息
Mol Brain. 2017 Feb 7;10(1):6. doi: 10.1186/s13041-017-0285-z.
Deregulation of α-synuclein encoding gene (SNCA) is one of the important facets of Parkinson's disease (PD) research. DNA methylation status of SNCA-intron1 has been shown to regulate the α-synuclein expression. The present study is aimed at investigating whether methylation of SNCA-intron1 is associated with higher expression of α-synuclein in PD. We have investigated the intron1 methylation status from 16 post-mortem brain samples comprised of 8 PD and 8 control subjects using bisulfite sequencing. We further correlated this methylation status with α-synuclein protein levels in substantia nigra of that individual using western blot analysis. We did not observe any significant difference in methylation of SNCA-intron1 region between PD and control samples. Moreover, no correlation was observed between methylation of SNCA-intron1 with α-synuclein level. Methylation of SNCA-intron1 region does not correlate with α-synuclein expression in PD samples.
α-突触核蛋白编码基因 (SNCA) 的去调控是帕金森病 (PD) 研究的重要方面之一。SNCA-intron1 的 DNA 甲基化状态已被证明可以调节 α-突触核蛋白的表达。本研究旨在探讨 SNCA-intron1 的甲基化是否与 PD 中 α-突触核蛋白的高表达有关。我们使用亚硫酸氢盐测序法研究了来自 16 个死后大脑样本的 intron1 甲基化状态，其中包括 8 个 PD 和 8 个对照样本。我们进一步使用 Western blot 分析，将该甲基化状态与个体黑质中的 α-突触核蛋白水平相关联。我们没有观察到 PD 和对照样本中 SNCA-intron1 区域的甲基化有任何显著差异。此外，SNCA-intron1 的甲基化与 α-突触核蛋白水平之间也没有观察到相关性。SNCA-intron1 区域的甲基化与 PD 样本中的 α-突触核蛋白表达不相关。
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