Giudice Maria Grazia, de Michele Francesca, Poels Jonathan, Vermeulen Maxime, Wyns Christine
Department of Gynecology-Andrology, Cliniques Universitaires Saint-Luc, Avenue Hippocrate 10, 1200 Brussels, Belgium.
Department of Gynecology-Andrology, Cliniques Universitaires Saint-Luc, Avenue Hippocrate 10, 1200 Brussels, Belgium; Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, Avenue Mounier 52, 1200 Brussels, Belgium.
Stem Cell Res. 2017 May;21:171-177. doi: 10.1016/j.scr.2017.01.009. Epub 2017 Feb 2.
Fertility preservation in prepubertal boys facing gonadotoxic treatment is still at the experimental stage. Nevertheless cryopreservation of immature testicular tissue (ITT) obtained by small testicular biopsy is being increasingly proposed in reproductive care clinics for this purpose. Different approaches to in vivo or in vitro mature spermatogonial stem cells (SSCs) contained in ITT have been studied: autografting of testicular tissue pieces, transplantation of one's own purified germ cell suspensions, and in vitro maturation (IVM) for subsequent use of sperm for intra cytoplasmic sperm injection (ICSI). While complete spermatogenesis yielding fertile offspring has been achieved in a number of animal species after cell and tissue transplantation and IVM, no mature sperm has yet been obtained from human prepubertal SSCs. This review describes research conducted by our team and a number of others working on fertility restoration from SSCs, with special emphasis on debated concerns and progress made towards clinical application of different strategies.
对于面临性腺毒性治疗的青春期前男孩，生育力保存仍处于实验阶段。然而，出于这一目的，生殖保健诊所越来越多地建议通过小睾丸活检获取未成熟睾丸组织（ITT）进行冷冻保存。人们已经研究了多种使ITT中包含的精原干细胞（SSC）在体内或体外成熟的方法：睾丸组织块自体移植、自身纯化生殖细胞悬液移植以及体外成熟（IVM）以便随后将精子用于胞浆内单精子注射（ICSI）。虽然在细胞和组织移植以及IVM后，许多动物物种已实现产生可育后代的完整精子发生，但尚未从人类青春期前的SSC中获得成熟精子。本综述描述了我们团队以及其他一些致力于从SSC恢复生育力的研究，特别强调了不同策略在临床应用方面存在争议的问题和取得的进展。
