Liebig V, Pollack E D
Institute for the Study of Developmental Disabilities, University of Illinois, Chicago 60680.
Anat Rec. 1989 Nov;225(3):218-23. doi: 10.1002/ar.1092250307.
Nerve fibers readily extend from larval frog spinal cord explants when cultured on a commercially available reconstituted basement-membrane-like matrix derived from the EHS mouse sarcoma. This superior, naturally derived substratum enables the in vitro development of complex neuritic arrays with long survival times in a defined medium, thus permitting the detailed analysis of spontaneous and target-mediated neuritogenesis.
当在源自EHS小鼠肉瘤的市售重组基底膜样基质上培养时，神经纤维很容易从幼蛙脊髓外植体延伸出来。这种优质的天然衍生基质能够在特定培养基中使复杂的神经突阵列在体外发育并具有较长的存活时间，从而允许对自发和靶标介导的神经突形成进行详细分析。
