Kuffler D P
Department of Pharmacology, Biocenter of the University, Basel, Switzerland.
J Comp Neurol. 1990 Dec 22;302(4):729-38. doi: 10.1002/cne.903020405.
Motoneurons of adult frog spinal cord have been retrogradely labeled with the carbocyanine derivative diI. Spinal cords were then dissociated and the labeled motoneurons partially purified by centrifugation over a bovine serum albumin (BSA) density gradient. The resulting cell suspension was plated on a substrate of innervated muscle extracellular matrix (ECM) to which the motoneurons attached and extended processes. Labeled adult motoneurons survived for more than 4 weeks in a defined medium in the absence of added serum or growth factors. These cultures of adult motoneurons provide a favorable preparation for studying molecular factors that influence process outgrowth and synapse formation.
成年青蛙脊髓的运动神经元已用羰花青衍生物二碘甲磺酸钠进行逆行标记。然后将脊髓解离，并通过在牛血清白蛋白（BSA）密度梯度上离心对标记的运动神经元进行部分纯化。将所得细胞悬液接种在由支配肌肉的细胞外基质（ECM）构成的底物上，运动神经元会附着于此并伸出突起。在没有添加血清或生长因子的限定培养基中，标记的成年运动神经元存活超过4周。这些成年运动神经元培养物为研究影响突起生长和突触形成的分子因素提供了良好的实验材料。
