Peng Zhong, Liang Wan, Liu Wenjing, Chen Huanchun, Wu Bin
State Key Laboratory of Agricultural Microbiology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, China.
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, China.
Arch Microbiol. 2017 May;199(4):635-640. doi: 10.1007/s00203-017-1341-x. Epub 2017 Feb 7.
Pasteurella multocida: subspecies multocida, gallicida, and septica are noted to have a contribution to fowl cholera, a life-threatening disease to both domestic and wild bird species. The genome sequences of avian P. multocida subspecies gallicida and multocida have been well analyzed and compared. However, the genome characterization of avian P. multocida subspecies septica is rarely discussed. In this study, we determined the draft genome sequence of a virulent P. multocida subspecies septica serogroup A strain HB02. The genome of P. multocida HB02 was composed of a single chromosome of 2,213,604 bp with a 40.27% G + C content, which showed a similar genome size and %GC content to the P. multocida subspecies multocida and gallicida genomes. The entire sequence specified 2002 putative coding DNA sequences plus 2 encoded rRNAs and 46 encoded tRNAs. In addition, the subspecies septica had a similar content of genes coding for metabolic traits that found in the subspecies gallicida and multocida. In addition, comparison of virulent versus avirulent avian P. multocida genomes identified 657 unique genes in either of the virulent strains HB02, P1059 and/or X73 compared to the avirulent strain Pm70. These genes should be the potential virulence-associated genes. Our work may add a novel genome sequence for the avian P. multocida genome database and shed a light on the pathogenesis of P. multocida.
多杀亚种、鸡亚种和败血亚种被认为与禽霍乱有关，禽霍乱对家禽和野生鸟类都是一种危及生命的疾病。禽多杀性巴氏杆菌鸡亚种和多杀亚种的基因组序列已得到充分分析和比较。然而，禽多杀性巴氏杆菌败血亚种的基因组特征却很少被讨论。在本研究中，我们确定了一株强毒多杀性巴氏杆菌败血亚种A血清型菌株HB02的基因组草图序列。多杀性巴氏杆菌HB02的基因组由一条2,213,604 bp的单染色体组成，G + C含量为40.27%，其基因组大小和%GC含量与多杀性巴氏杆菌多杀亚种和鸡亚种的基因组相似。整个序列共确定了2002个推定的编码DNA序列，外加2个编码的rRNA和46个编码的tRNA。此外，败血亚种编码代谢性状的基因含量与鸡亚种和多杀亚种相似。此外，对强毒与无毒禽多杀性巴氏杆菌基因组的比较发现，与无毒菌株Pm70相比，强毒株HB02、P1059和/或X73中的任何一个都有657个独特基因。这些基因应该是潜在的毒力相关基因。我们的工作可能会为禽多杀性巴氏杆菌基因组数据库增添一条新的基因组序列，并为多杀性巴氏杆菌的发病机制提供线索。
