Ma T, Xu L, Wang H, Guo X, Li Z, Wan F, Chen J, Liu L, Liu X, Chang G, Chen G
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, 225125, China.
Anim Genet. 2017 Jun;48(3):303-314. doi: 10.1111/age.12533. Epub 2017 Feb 7.
Salmonella enterica ser. Pullorum is one of the most easily re-infecting pathogens in poultry production because of its mechanism of escaping from immune elimination. We used the transcriptome method to investigate the variation in gene expression in chicken spleen resulting from the interaction between hosts and S. Pullorum in the survival process. The expression of various genes related to the maturation and activation of B cells was activated before S. Pullorum was eliminated, which might help S. Pullorum escape from the elimination process. The suppression of some genes involved in the fusion of autophagosomes and lysosomes, such as MYO6, was identified and may be regulated by the secretion systems of S. Pullorum. In addition, a large proportion of these differentially expressed genes could be localized in the identified quantitative trait loci regions associated with the antibody response to bacteria. Collectively, these identified genes provided an outline for further understanding the interaction between chicken immune cells and S. Pullorum in chicken spleen.
鸡白痢沙门氏菌是家禽生产中最易再次感染的病原体之一，因其具有逃避免疫清除的机制。我们采用转录组方法研究宿主与鸡白痢沙门氏菌在存活过程中相互作用导致鸡脾脏基因表达的变化。在鸡白痢沙门氏菌被清除之前，与B细胞成熟和激活相关的各种基因的表达被激活，这可能有助于鸡白痢沙门氏菌逃避清除过程。我们鉴定出一些参与自噬体与溶酶体融合的基因（如MYO6）受到抑制，这可能受鸡白痢沙门氏菌分泌系统的调控。此外，这些差异表达基因中的很大一部分可定位在与细菌抗体反应相关的已鉴定数量性状位点区域。总体而言，这些已鉴定的基因提供了一个框架，有助于进一步了解鸡脾脏中鸡免疫细胞与鸡白痢沙门氏菌之间的相互作用。
