Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China.
Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Science, 2901 Beidi Road, 201106, Shanghai, China.
Anim Genet. 2019 Aug;50(4):403-406. doi: 10.1111/age.12787. Epub 2019 Apr 24.
Pullorum is a bacterial disease that threatens the modern poultry industry. Over the years, research on this topic has focused mainly on its epidemiology, whereas the hosts' genetic basis of infection is still vague. In order to identify chickens' genes associated with pullorum, we sequenced 300 New Pudong chicken by double digest genotyping-by-sequencing. We obtained 1 527 953 SNPs for a genome-wide association analysis, which identified 43 genome-wide significant markers. Most of the significant SNPs were in the interval of 57.7-59.0 Mb on chromosome 5. The gene set enrichment analysis suggests a potential manner for bacterial infection and remaining inside the host. This work provides basic data for the purification, prevention and treatment of pullorum disease.
鸡白痢是一种威胁现代家禽业的细菌性疾病。多年来，该主题的研究主要集中在其流行病学方面，而宿主感染的遗传基础仍不清楚。为了鉴定与鸡白痢相关的基因，我们通过双酶切测序对 300 只新浦东鸡进行了测序。我们获得了 1527953 个 SNPs 进行全基因组关联分析，鉴定出 43 个全基因组显著标记。大多数显著 SNP 位于 5 号染色体上的 57.7-59.0 Mb 区间内。基因集富集分析表明了细菌感染和在宿主内潜伏的潜在方式。这项工作为鸡白痢病的净化、预防和治疗提供了基础数据。
