玉米（Zea mays L.）中一种新型花粉特异性启动子的分离与鉴定。

Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.).

作者信息

Wang He, Fan Mingxia, Wang Guohong, Zhang Chunyu, Shi Lei, Wei Zhengyi, Ma Wenjuan, Chang Jing, Huang Senxin, Lin Feng

机构信息

a Agronomy College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.

b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.

出版信息

Genome. 2017 Jun;60(6):485-495. doi: 10.1139/gen-2016-0089. Epub 2017 Jan 4.

DOI:10.1139/gen-2016-0089

PMID:28177828

Abstract

ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.

摘要

ZmSTK2_USP位于玉米4号染色体的长臂上，属于玉米中的丝氨酸/苏氨酸激酶基因。对起始密码子ATG上游2100 bp的序列分析表明，它含有顺式作用元件基序以及两种花药/花粉特异性启动子元件（GTGA和AGAAA），这表明它是花粉特异性启动子。为了研究ZmSTK2_USP启动子的功能，采用了GUS基因融合系统。在proZmSTK2_USP-GUS转基因植物中，在成熟花粉粒和花粉管中检测到了GUS活性，但在其他花器官和营养组织中未检测到。这些结果表明，proZmSTK2_USP是花粉特异性启动子，并在花粉发育中期直至花粉成熟期间驱动花粉特异性活性。