Hamilton D A, Schwarz Y H, Mascarenhas J P
Department of Biological Sciences and Center for Molecular Genetics, University at Albany, State University of New York, 12222, USA.
Plant Mol Biol. 1998 Nov 1;38(4):663-9. doi: 10.1023/a:1006083725102.
The region of the promoter of the pollen-specific maize gene, ZM13, from -119 to -37 was analyzed by a linker-scanning type of substitution mutagenesis and two areas were shown to be responsible for pollen expression: a proximal region delineated by mutations from -84 to -53 that conferred pollen specificity, and an upstream region delineated by a mutation from -107 to -102 (Q-element) that could increase the expression of the proximal region but showed no ability to cause expression in pollen on its own. Replacement of both of these areas with other sequences including the CaMV 35S promoter failed to replace activity.
通过连接子扫描类型的取代诱变分析了花粉特异性玉米基因ZM13启动子从-119至-37的区域，结果表明有两个区域负责花粉表达：一个近端区域，由-84至-53的突变界定，赋予花粉特异性；另一个上游区域，由-107至-102的突变（Q元件）界定，它可以增加近端区域的表达，但自身没有在花粉中引发表达的能力。用包括CaMV 35S启动子在内的其他序列替换这两个区域均未能取代其活性。
