Moulinoux J P, Quemener V, Khan N A, Delcros J G, Havouis R
Groupe de Recherche en Thérapeutique Anticancereuse, CNRS SDI, C.H.U. de Rennes, France.
Anticancer Res. 1989 Jul-Aug;9(4):1057-62.
High red blood cell (RBC) spermidine levels have been observed in patients harboring various histological types of cancer. In an attempt to explain the mechanism of RBC spermidine uptake in the malignant cell growth process, erythrocytes from normal and (3LL) Lewis lung carcinoma grafted mice were incubated with [14C] spermidine in the presence of PBS or plasma from normal or 3LL grafted mice. Though RBC of cancer mice harbor abnormally elevated spermidine concentrations as compared with those of controls, when incubated with PBS, 3LL grafted mice exhibit [14C] spermidine uptake three times higher than that of normal mice erythrocytes in the same incubating conditions. Moreover, if plasma incubations always increase spermidine uptake in RBC from cancer mice compared with erythrocytes only incubated with PBS, plasma incubations are responsible for two opposite effects in RBC of normal mice: plasma increases spermidine uptake in PBS unwashed erythrocytes and lowers it in PBS washed RBC. One possible explanation for the observed plasma stimulating effect in RBC spermidine uptake might be the presence of a plasma component common to plasma from normal and cancer mice, able to interact with RBC membrane proteins. In erythrocytes from cancer mice, the high spermidine concentration would be responsible for the observed compactness in band 3 proteins involved in ionic transport through RBC stroma. In normal mice erythrocytes which exhibit low polyamine levels, the observed dissociation of band 3 proteins might explain the spermidine uptake in PBS washed normal RBC incubated with plasma. In vivo, this plasma component would participate in the stabilization of normal mice membrane proteins involved in RBC spermidine uptake.
在患有各种组织学类型癌症的患者中，已观察到红细胞（RBC）中的亚精胺水平较高。为了解释恶性细胞生长过程中红细胞摄取亚精胺的机制，将来自正常小鼠和移植了（3LL）Lewis肺癌的小鼠的红细胞与[14C]亚精胺在正常小鼠或移植了3LL的小鼠的磷酸盐缓冲液（PBS）或血浆存在下进行孵育。尽管与对照组相比，癌症小鼠的红细胞中亚精胺浓度异常升高，但在相同孵育条件下，当与PBS孵育时，移植了3LL的小鼠的红细胞对[14C]亚精胺的摄取比正常小鼠红细胞高两倍。此外，如果血浆孵育总是使癌症小鼠红细胞中的亚精胺摄取量高于仅与PBS孵育的红细胞，那么血浆孵育对正常小鼠红细胞会产生两种相反的影响：血浆会增加未用PBS洗涤的红细胞中亚精胺的摄取量，并降低用PBS洗涤的红细胞中亚精胺的摄取量。观察到的血浆对红细胞摄取亚精胺的刺激作用的一种可能解释可能是正常小鼠和癌症小鼠血浆中存在一种共同的血浆成分，能够与红细胞膜蛋白相互作用。在癌症小鼠的红细胞中，高浓度的亚精胺可能是导致参与通过红细胞基质进行离子转运的带3蛋白紧密性增加的原因。在多胺水平较低的正常小鼠红细胞中，观察到的带3蛋白解离可能解释了与血浆孵育的经PBS洗涤的正常红细胞中亚精胺的摄取。在体内，这种血浆成分将参与稳定参与红细胞摄取亚精胺的正常小鼠膜蛋白。
