Tse Wai Hei, Gyenis Laszlo, Litchfield David W, Zhang Jin
Department of Chemical and Biochemical Engineering, Western University, London, Ontario, Canada.
J Biomater Appl. 2017 Feb;31(7):1087-1096. doi: 10.1177/0885328216684651. Epub 2016 Dec 15.
Theranostic applications of gelatin nanospheres require two major components, a method of detection and good biocompatibility. We characterized the response of UTA-6 human osteosarcoma cells to the introduction of functionalized 90 bloom-based gelatin nanospheres (158 ± 49 nm) modified with three elements in different order: (a) hybridization with cadmium-based quantum dots for optical detection, (b) bioconjugation with anti-human IgG FAB (anti-IgG) for cell targeting, with/without (c) capping with polyethylene glycol on the surface for enhanced biocompatibility. A one-pot process is developed for incorporating quantum dots and antibody with gelatin nanospheres. Path A of modifying gelatin nanospheres with quantum dots first followed by anti-IgG resulted in a significantly greater cellular viability than Path B with anti-IgG first followed by quantum dots. Capping with polyethylene glycol as the final step in modification yielded significantly opposing results with decreases in Path A and increases in Path B. Three-dimensional z-stacking fluorescent images of hybrid gelatin nanospheres with anti-IgG is observed to have an increase in cellular association. The observed results suggest the modification order for building hybrid nanospheres may have an impact on cellular response.
明胶纳米球的诊疗应用需要两个主要组成部分，即检测方法和良好的生物相容性。我们表征了UTA-6人骨肉瘤细胞对引入用三种元素以不同顺序修饰的功能化90 Bloom级明胶纳米球（158±49纳米）的反应：(a) 与镉基量子点杂交用于光学检测，(b) 与抗人IgG FAB（抗IgG）生物共轭用于细胞靶向，有/无 (c) 在表面用聚乙二醇封端以增强生物相容性。开发了一种将量子点和抗体与明胶纳米球结合的一锅法。先用量子点修饰明胶纳米球然后再用抗IgG的路径A，比先用抗IgG然后再用量子点的路径B具有显著更高的细胞活力。在修饰的最后一步用聚乙二醇封端产生了显著相反的结果，路径A中细胞活力降低，路径B中细胞活力增加。观察到与抗IgG的混合明胶纳米球的三维z堆叠荧光图像显示细胞结合增加。观察结果表明构建混合纳米球的修饰顺序可能对细胞反应有影响。
