Zhu Xiali, Huang Heqing, Zhang Yingjie, Zhang Huijuan, Hou Lin, Zhang Zhenzhong
1 School of Pharmaceutical Sciences, Henan University of Chinese Medicine, Zhengzhou, PR China.
2 School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, PR China * Both the authors contributed equally to this work.
J Biomater Appl. 2017 Feb;31(7):1010-1025. doi: 10.1177/0885328216676159. Epub 2016 Oct 26.
Safe and efficient drug delivery in a controllable fashion, especially remote and repeatable switch of on-demand drug release, is the subject of widespread attention. A kind of magnetic nanoparticles (DOX-Cit/CuS@FeO-NPs) simultaneously consisted of Cit/CuS@FeO and doxorubicin (DOX) was presented. The drug release from DOX-Cit/CuS@FeO-NPs could be successfully triggered by the presence of gelatinase, showing great promise for tumor-targeted drug release through an enzymatic degradation mechanism. Compared with free DOX, DOX-Cit/CuS@FeO-NPs could not only specially deliver Cit/CuS@FeO and DOX into MCF-7 cells, but also could greatly improve the quantity of ROS produced in MCF-7 cells under of 980 nm laser irradiation. DOX-Cit/CuS@FeO-NPs also had highly selective accumulation at tumor tissue of S tumor-bearing mice, which were along with a magnet near the tumor site. Furthermore, when combined with NIR laser irridation, DOX-Cit/CuS@FeO-NPs showed a higher antitumor efficacy than the individual therapies in vitro and in vivo. This study showed that DOX-Cit/CuS@FeO-NPs could be used as a platform for tumor chemotherapy, photothermal and photodynamic therapy.
以可控方式实现安全高效的药物递送，尤其是按需药物释放的远程和可重复切换，是广受关注的课题。本文提出了一种同时由Cit/CuS@FeO和阿霉素（DOX）组成的磁性纳米颗粒（DOX-Cit/CuS@FeO-NPs）。DOX-Cit/CuS@FeO-NPs的药物释放可通过明胶酶的存在成功触发，这表明通过酶降解机制实现肿瘤靶向药物释放具有很大潜力。与游离DOX相比，DOX-Cit/CuS@FeO-NPs不仅能将Cit/CuS@FeO和DOX特异性递送至MCF-7细胞，还能在980 nm激光照射下显著提高MCF-7细胞中产生的活性氧数量。DOX-Cit/CuS@FeO-NPs在携带S肿瘤的小鼠肿瘤组织中也有高度选择性积累，这些小鼠在肿瘤部位附近放置了一块磁铁。此外，当与近红外激光照射相结合时，DOX-Cit/CuS@FeO-NPs在体外和体内均显示出比单一疗法更高的抗肿瘤疗效。这项研究表明，DOX-Cit/CuS@FeO-NPs可作为肿瘤化疗、光热和光动力治疗的平台。
