Sinha Meenal, Lowell Clifford A
Department of Laboratory Medicine and the Program in Immunology, University of California, San Francisco, USA.
Bio Protoc. 2016 Nov 20;6(22). doi: 10.21769/BioProtoc.2013.
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for EpCAM, a pan-epithelial cell marker. This method yields 2-3 × 10 ATII cells per mouse lung. The cell preps are highly pure and viable and can be used for genomic or proteomic analyses or cultured to understand their roles in various biological processes.
在本方案中，我们描述了从未经处理的小鼠肺中分离高纯度原代II型肺泡上皮细胞（ATII）的方法。该方法结合了对多种谱系标志物的阴性选择以及对全上皮细胞标志物EpCAM的阳性选择。此方法每只小鼠肺可产生2 - 3×10个ATII细胞。细胞制备物高度纯净且具有活力，可用于基因组或蛋白质组分析，或进行培养以了解它们在各种生物学过程中的作用。
