非经典环核苷酸的质谱分析

Mass Spectrometric Analysis of Non-canonical Cyclic Nucleotides.

作者信息

Bähre Heike, Kaever Volkhard

机构信息

Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

Research Core Unit Metabolomics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

出版信息

Handb Exp Pharmacol. 2017;238:293-306. doi: 10.1007/164_2016_5001.

DOI:10.1007/164_2016_5001

PMID:28181004

Abstract

Contemporary investigations regarding the (patho)physiological roles of the non-canonical cyclic nucleoside monophosphates (cNMP) cytidine 3',5'-cyclic monophosphate (cCMP) and uridine 3',5'-cyclic monophosphate (cUMP) have been hampered by the lack of highly specific and sensitive analytic methods for these analytes. In addition, the existence of 2',3'-cNMP besides 3',5'-cNMP has been described recently. HPLC coupled with tandem mass spectrometry (HPLC-MS/MS) is the method of choice for identification and quantification of low-molecular weight endogenous metabolites. In this chapter, recommendations for an HPLC-MS/MS method for 3',5'- and 2',3'-cNMP are summarized.

摘要

关于非经典环核苷单磷酸（cNMP）胞苷3'，5'-环单磷酸（cCMP）和尿苷3'，5'-环单磷酸（cUMP）的（病理）生理作用，当代研究因缺乏针对这些分析物的高特异性和高灵敏度分析方法而受阻。此外，最近已报道除了3'，5'-cNMP外还存在2'，3'-cNMP。高效液相色谱-串联质谱联用（HPLC-MS/MS）是鉴定和定量低分子量内源性代谢物的首选方法。在本章中，总结了一种用于3'，5'-和2'，3'-cNMP的HPLC-MS/MS方法的建议。