抗L1细胞粘附分子自身抗体的循环水平作为食管鳞状细胞癌潜在的诊断生物标志物
Circulating levels of autoantibodies against L1-cell adhesion molecule as a potential diagnostic biomarker in esophageal squamous cell carcinoma.
作者信息
Xu Y-W, Peng Y-H, Ran L-Q, Zhai T-T, Guo H-P, Qiu S-Q, Chen H-L, Wu Z-Y, Li E-M, Xie J-J
机构信息
Department of Clinical Laboratory Medicine, The Cancer Hospital of Shantou University Medical College, Shantou, China.
The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College, Shantou, China.
出版信息
Clin Transl Oncol. 2017 Jul;19(7):898-906. doi: 10.1007/s12094-017-1623-4. Epub 2017 Feb 8.
BACKGROUND
Esophageal squamous cell carcinoma (ESCC) is a common malignant disease worldwide, especially in China. We aimed to determine the level of autoantibodies against L1CAM in patients with ESCC.
METHODS
Levels of circulating autoantibodies against L1CAM antigens were determined by an enzyme-linked immunosorbent assay in cohort 1 (191 patients with ESCC and 94 normal controls) and validated in cohort 2 (47 patients with ESCC and 47 normal controls). Receiver-operating characteristics were employed to calculate diagnostic accuracy. Cumulative survival time was calculated by the Kaplan-Meier method and analyzed by the log-rank test.
RESULTS
In cohorts 1 and 2, levels of autoantibodies against L1CAM were all significantly higher in sera of patients with ESCC compared to normal controls (P < 0.05). Detection of autoantibodies against L1CAM provided a sensitivity of 26.2%, a specificity of 90.4%, and an area under the curve (AUC) of 0.603 (95% CI 0.535-0.672) in diagnosing ESCC in cohort 1, and a sensitivity of 27.7%, a specificity of 91.5%, and an AUC of 0.628 (95% CI 0.516-0.741). Similar results were observed in the diagnosis of early stage ESCC (25.2% sensitivity, 90.4% specificity, and an AUC of 0.611 (95% CI 0.533-0.689) in cohort 1, and 33.3% sensitivity, 91.5% specificity, and an AUC of 0.636 (95% CI 0.439-0.832) in cohort 2). Moreover, positive rates of autoantibodies against L1CAM had no statistical correlation with clinical outcome of ESCC (P > 0.05).
CONCLUSIONS
Our results suggest that circulating autoantibodies against L1CAM is a potential biomarker for the early detection of ESCC.
背景
食管鳞状细胞癌（ESCC）是一种全球常见的恶性疾病，在中国尤为常见。我们旨在确定ESCC患者中抗L1细胞黏附分子（L1CAM）自身抗体的水平。
方法
在队列1（191例ESCC患者和94例正常对照）中通过酶联免疫吸附测定法测定抗L1CAM抗原的循环自身抗体水平，并在队列2（47例ESCC患者和47例正常对照）中进行验证。采用受试者操作特征曲线来计算诊断准确性。采用Kaplan-Meier方法计算累积生存时间，并通过对数秩检验进行分析。
结果
在队列1和队列2中，ESCC患者血清中抗L1CAM自身抗体水平均显著高于正常对照（P<0.05）。在队列1中，检测抗L1CAM自身抗体诊断ESCC的灵敏度为26.2%，特异度为90.4%，曲线下面积（AUC）为0.603（95%CI 0.535 - 0.672）；在队列2中，灵敏度为27.7%，特异度为91.5%，AUC为0.628（95%CI 0.516 - 0.741）。在早期ESCC诊断中观察到类似结果（队列1中灵敏度为25.2%，特异度为90.4%，AUC为0.611（95%CI 0.533 - 0.689）；队列2中灵敏度为33.3%，特异度为91.5%，AUC为0.636（95%CI 0.439 - 0.832））。此外，抗L1CAM自身抗体阳性率与ESCC临床结局无统计学相关性（P>0.05）。
结论
我们的结果表明，循环抗L1CAM自身抗体是ESCC早期检测的潜在生物标志物。
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