血清 L1 细胞黏附分子在食管鳞状细胞癌中的诊断和预后价值。
Diagnostic and prognostic value of serum L1-cell adhesion molecule in esophageal squamous cell carcinoma.
机构信息
Department of Clinical Laboratory Medicine, The Cancer Hospital of Shantou University Medical College, Shantou 515041, PR China; Department of Biochemistry and Molecular Biology, Shantou University Medical College, No. 22, Xinling road, Shantou 515041, PR China.
Cancer Research Lab, The Cancer Hospital of Shantou University Medical College, Shantou 515041, PR China.
出版信息
Clin Res Hepatol Gastroenterol. 2018 Dec;42(6):597-603. doi: 10.1016/j.clinre.2018.08.010. Epub 2018 Sep 13.
OBJECTIVE
L1 cell adhesion molecule (L1CAM) has been found to be dysregulated in several types of human cancers. Here, we aimed to determine the level of soluble L1CAM in serum of patients with esophageal squamous cell carcinoma (ESCC).
METHODS
Serum levels of L1CAM were determined by an enzyme-linked immunosorbent assay (ELISA) in 191 patients with ESCC and 94 normal controls. Receiver operating characteristics (ROC) was employed to calculate diagnostic accuracy. Cumulative survival time was calculated by the Kaplan-Meier method and analyzed by the logrank test.
RESULTS
Levels of L1CAM were significantly lower in all ESCC patients than in normal controls (P < 0.001). Detection of serum L1CAM provided a sensitivity of 28.3%, a specificity of 90.4% and an area under the curve (AUC) of 0.644 (95% CI: 0.579-0.710) in diagnosing ESCC. Similar results were observed in the diagnosis of early-stage ESCC (26.2% sensitivity, 90.4% specificity, and an AUC of 0.629). Moreover, decreased level of L1CAM was correlated with depth of tumor invasion (P < 0.05). Kaplan-Meier analysis showed that lower serum L1CAM level was significantly related to shorter overall survival time (P = 0.036) and disease-free survival time (P = 0.021) of ESCC patients.
CONCLUSIONS
Our study demonstrated that serum L1CAM might serve as a potential biomarker for the diagnosis and prognosis of ESCC.
目的
L1 细胞黏附分子（L1CAM）在多种人类癌症中失调。本研究旨在测定食管鳞状细胞癌（ESCC）患者血清中可溶性 L1CAM 的水平。
方法
采用酶联免疫吸附试验（ELISA）检测 191 例 ESCC 患者和 94 例正常对照者血清中 L1CAM 的水平。采用受试者工作特征（ROC）曲线计算诊断准确性。采用 Kaplan-Meier 法计算累积生存时间，并采用 logrank 检验进行分析。
结果
所有 ESCC 患者的 L1CAM 水平均明显低于正常对照组（P<0.001）。检测血清 L1CAM 对 ESCC 的诊断灵敏度为 28.3%，特异度为 90.4%，曲线下面积（AUC）为 0.644（95%CI：0.579-0.710）。在诊断早期 ESCC 时也观察到了相似的结果（灵敏度为 26.2%，特异度为 90.4%，AUC 为 0.629）。此外，L1CAM 水平的降低与肿瘤浸润深度相关（P<0.05）。Kaplan-Meier 分析显示，血清 L1CAM 水平较低与 ESCC 患者总生存时间（P=0.036）和无病生存时间（P=0.021）较短显著相关。
结论
本研究表明，血清 L1CAM 可能作为 ESCC 诊断和预后的潜在生物标志物。
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