Ansari Niha, Menon Shobhana K
Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat, 380009, India.
J Forensic Sci. 2017 Sep;62(5):1351-1356. doi: 10.1111/1556-4029.13430. Epub 2017 Feb 9.
Determination of time since death (TSD) plays very important role in forensic examination as it narrows down field of suspects and aids in deceased identification. This study utilizes the fluorescence property of vitreous humor (VH) tryptophan to determine TSD using o-phthalaldehyde (OPA). The detection limit of these fluorometric studies was found to be 8 ppb indicating sensitivity and high accuracy in TSD determination. The study was performed on selected 76 cadaver with known TSD ranging from 3 to 90 h. Excellent correlation between VH tryptophan and TSD was obtained with a coefficient of correlation R = 0.9590. Results showed statistically significant increase in vitreous tryptophan with TSD up to 90 h, and the proposed method was efficaciously applied for prediction of TSD as no systematic error exist. The regression equation obtained from the study is [Trp] = 2.21 + 2.98 * TSD.
死后时间（TSD）的确定在法医检查中起着非常重要的作用，因为它可以缩小嫌疑人范围并有助于死者身份识别。本研究利用玻璃体液（VH）色氨酸的荧光特性，使用邻苯二甲醛（OPA）来确定TSD。这些荧光研究的检测限为8 ppb，表明在TSD测定中具有灵敏度和高精度。该研究在选定的76具已知TSD范围为3至90小时的尸体上进行。VH色氨酸与TSD之间具有良好的相关性，相关系数R = 0.9590。结果显示，直至90小时，玻璃体液色氨酸随TSD呈统计学上的显著增加，并且由于不存在系统误差，所提出的方法有效地应用于TSD的预测。从该研究中获得的回归方程为[Trp] = 2.21 + 2.98 * TSD。
