Zhang Kangkang, Pei Yali, Gan Zhihao, Zhang Xuetao, Duan Ye, Liu Yuepeng, Liu Wei
From the *Department of Anesthesiology, Tengzhou Central People's Hospital, China; †Department of Medical Administration, Tengzhou Central People's Hospital, China; and ‡Center for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, China.
J Burn Care Res. 2017 Sep/Oct;38(5):e842-e850. doi: 10.1097/BCR.0000000000000502.
The objective of this study was to develop rat model of second-degree burn pain and test analgesic efficacy of local thiamine administration. Automatic temperature-controlled hot plate was set at 85 ± 0.1°C with a filter paper on the top. Rats were thrust on hot plate landing on plantar surface for 4 to 7 and 10 seconds, respectively. Burnt skin was observed. Hematoxylin and eosin staining and Masson staining were used to monitor burn degree. Gait analysis detected change of locomotion. Allodynia and hyperalgesia in the burnt area were evaluated with von Frey test and Hargreaves Test, and ongoing pain was detected with conditional place preference test. Markers for the activity of microglia (Iba1), astrocytes (GFAP), and neurons (c-fos) were detected with immunofluorescence. Finally, thiamine was injected into blisters to observe its effect on burn pain. Blisters on burnt skin, space between dermal and epidermal layers in hematoxylin and eosin staining and burn injury limiting in dermal layer in Masson stain all indicated that burn injury lasting for 7 seconds matched second-degree burn. Behavioral tests revealed allodynia, ongoing pain, and increased expression of c-fos, GFAP, and Iba1, as well as the absence of hyperalgesia in Burn7s. Burn injury reduced distance of second and fourth digits. MK801 could relieve allodynia in Burn7s. Local administration of 1, 2, and 4 mg of thiamine had no effect on the allodynia, but 2 and 4 mg of thiamine also could induce conditional place preference (CPP) in Burn7s. A rat model of second-degree burn pain was developed and local administration of thiamine provided relief from pain.
本研究的目的是建立大鼠二度烧伤疼痛模型，并测试局部注射硫胺素的镇痛效果。自动控温热板设定为85±0.1°C，顶部放置一张滤纸。将大鼠分别在热板上足底接触4至7秒和10秒。观察烧伤皮肤情况。采用苏木精-伊红染色和Masson染色监测烧伤程度。步态分析检测运动变化。用von Frey试验和哈格里夫斯试验评估烧伤区域的痛觉过敏和异常性疼痛，并用条件性位置偏爱试验检测持续性疼痛。通过免疫荧光检测小胶质细胞（Iba1）、星形胶质细胞（GFAP）和神经元（c-fos）活性的标志物。最后，将硫胺素注入水疱中观察其对烧伤疼痛的影响。烧伤皮肤出现水疱、苏木精-伊红染色显示真皮和表皮层之间有间隙以及Masson染色显示烧伤损伤局限于真皮层，均表明持续7秒的烧伤符合二度烧伤。行为学测试显示Burn7s组存在痛觉过敏、持续性疼痛，c-fos、GFAP和Iba1表达增加，且无痛觉过敏。烧伤损伤缩短了第二和第四趾的间距。MK801可缓解Burn7s组的痛觉过敏。局部注射1、2和4mg硫胺素对痛觉过敏无影响，但2和4mg硫胺素也可在Burn7s组诱导条件性位置偏爱（CPP）。建立了大鼠二度烧伤疼痛模型，局部注射硫胺素可缓解疼痛。
