Blüher Susann, Käpplinger Jakob, Herget Sabine, Reichardt Sandra, Böttcher Yvonne, Grimm Andrea, Kratzsch Jürgen, Petroff David
Integrated Research and Treatment Center (IFB) for AdiposityDiseases, University of Leipzig, Germany; Department of Pediatrics, University Hospital of Halle-Wittenberg, Halle, Germany.
Integrated Research and Treatment Center (IFB) for AdiposityDiseases, University of Leipzig, Germany.
Metabolism. 2017 Mar;68:77-87. doi: 10.1016/j.metabol.2016.11.015. Epub 2016 Dec 11.
The impact of high-intensity interval training (HIIT) as well as the association between the adipocyte fatty binding protein (aFABP) and cardiometabolic risk factors in overweight adolescents was investigated.
Twenty-eight adolescents (13-18years; BMI≥90th percentile according to German reference values) were offered HIIT twice weekly for 6months. At baseline and after program completion, anthropometric, clinical and metabolic characteristics were assessed and a fasting blood sample was obtained. Leptin, adiponectin, visfatin and aFABP were measured using commercially available kits. DNA methylation at RALBP1 was assessed using pyrosequencing. Descriptive statistics, Pearson's correlation and linear models were calculated.
Mean age at start of the program was 15.5±1.4years (53.5% females) and 20/28 (71%) provided follow-up data. At baseline, aFABP was correlated with BMI-SDS (0.48 [0.13,0.72]; p=0.0095), waist-to-height-ratio (0.63 [0.33,0.81], p=0.00036) and body fat content (0.55 [0.21, 0.77]; p=0.0031). Certain markers of metabolic risk were significantly correlated with aFABP (HOMA-IR 0.52 [0.19, 0.75], p=0.0044; γGT 0.48 [0.13, 0.73], p=0.0091; uric acid 0.46 [0.11, 0.71] p=0.013; HDL-C -0.39 [-0.66, -0.01] p=0.043; triglycerides 0.38 [0.01, 0.66], p=0.047). With the exception of triglycerides, these associations vanished after adjusting for BMI-SDS. aFABP did not depend on sex, age or pubertal stage in obese adolescents. After the HIIT program, small but significant reductions were observed in waist-to-height-ratio, (0.013 [0.0025, 0.024]; p=0.023), skin-fold based body fat content (2.0% [0.6, 3.5]; p=0.011), and standard deviation score of systolic blood pressure (0.69 [0.26 to 1.1]; p=0.0036). No changes were observed in adipokines or epigenetic markers following the program.
HIIT may have beneficial effects on body composition and cardiometabolic health in overweight adolescents. Like in adults, aFABP seems to be associated with markers of metabolic risk in obese adolescents.
研究高强度间歇训练（HIIT）对超重青少年的影响以及脂肪细胞脂肪酸结合蛋白（aFABP）与心血管代谢危险因素之间的关联。
28名青少年（13 - 18岁；根据德国参考值，BMI≥第90百分位数）每周进行两次HIIT，共6个月。在基线和项目结束后，评估人体测量、临床和代谢特征，并采集空腹血样。使用市售试剂盒检测瘦素、脂联素、内脂素和aFABP。采用焦磷酸测序法评估RALBP1的DNA甲基化。计算描述性统计量、Pearson相关性和线性模型。
项目开始时的平均年龄为15.5±1.4岁（53.5%为女性），28人中有20人（71%）提供了随访数据。在基线时，aFABP与BMI标准差（0.48 [0.13,0.72]；p = 0.0095）、腰高比（0.63 [0.33,0.81]，p = 0.00036）和体脂含量（0.55 [0.21, 0.77]；p = 0.0031）相关。某些代谢风险标志物与aFABP显著相关（HOMA-IR 0.52 [0.19, 0.75]，p = 0.0044；γGT 0.48 [0.13, 0.73]，p = 0.0091；尿酸0.46 [0.11, 0.71] p = 0.013；HDL-C -0.39 [-0.66, -0.01] p = 0.043；甘油三酯0.38 [0.01, 0.66]，p = 0.047）。除甘油三酯外，在调整BMI标准差后，这些关联消失。肥胖青少年的aFABP不依赖于性别、年龄或青春期阶段。HIIT项目后，腰高比（0.013 [0.0025, 0.024]；p = 0.023）、基于皮褶厚度的体脂含量（2.0% [0.6, 3.5]；p = 0.011）和收缩压标准差（0.69 [0.26至1.1]；p = 0.0036）出现了小幅度但显著的降低。项目后未观察到脂肪因子或表观遗传标志物的变化。
HIIT可能对超重青少年的身体成分和心血管代谢健康有益。与成年人一样，aFABP似乎与肥胖青少年的代谢风险标志物相关。
