Baran Nuray Yılmaz, Acet Ömür, Odabaşı Mehmet
Department of Chemistry, Faculty of Science and Arts, Aksaray University, Aksaray, Turkey.
Department of Chemistry, Faculty of Science and Arts, Aksaray University, Aksaray, Turkey.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:15-20. doi: 10.1016/j.msec.2016.12.036. Epub 2016 Dec 13.
In this study, a supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based Cu-attached bentonite particles embedded hybrid monolithic cryogel (Cu-ABPs EHMC) was prepared by radical cryo-copolymerization of HEMA with N,N'-methylene-bis-acrylamide (MBAAm) as a cross-linker directly in a plastic syringe in the presence of Cu-ABPs, and used for hemoglobin (Hb) separation from aqueous solution. Due to the naturally abundant hydroxyl groups on bentonite, Cu-attached bentonite particles (Cu-ABPs) embedded hybrid monolithic cryogel (HMC) showed a good adsorption performance for Hb, covering an excellent adsorption capacity of 521.6mg/g bentonite in phosphate buffer at pH6.0 with a flow rate of 0.5mL/min, fast binding stability within 60min, well selectivity and reversibility. Compared with other adsorbent systems used for protein separation, this sorbent has many advantages such as excellent adsorption performance, ease of preparation, suitable, effective and environment-friendly to perform, which can serve as a more sustainable material in protein separation and purification.
在本研究中，通过在塑料注射器中，以N，N'-亚甲基双丙烯酰胺（MBAAm）作为交联剂，在Cu附着膨润土颗粒（Cu-ABPs）存在下，使甲基丙烯酸2-羟乙酯（HEMA）进行自由基冷冻共聚，制备了一种基于超大孔聚甲基丙烯酸2-羟乙酯（PHEMA）的附着Cu的膨润土颗粒嵌入的混合整体式冷冻凝胶（Cu-ABPs EHMC），并将其用于从水溶液中分离血红蛋白（Hb）。由于膨润土上天然存在丰富的羟基，附着Cu的膨润土颗粒（Cu-ABPs）嵌入的混合整体式冷冻凝胶（HMC）对Hb表现出良好的吸附性能，在pH6.0的磷酸盐缓冲液中，流速为0.5mL/min时，膨润土的吸附容量高达521.6mg/g，在60min内具有快速的结合稳定性、良好的选择性和可逆性。与其他用于蛋白质分离的吸附剂系统相比，这种吸附剂具有许多优点，如优异的吸附性能、易于制备、适用、有效且环保，可作为蛋白质分离和纯化中更具可持续性的材料。
