Chappel R J, Millar B D, Adler B, Hill J, Jeffers M J, Jones R T, McCaughan C J, Mead L J, Skilbeck N W
Department of Agriculture and Rural Affairs, Veterinary Research Institute Attwood and Parkville, Victoria.
Aust Vet J. 1989 Oct;66(10):330-3. doi: 10.1111/j.1751-0813.1989.tb09719.x.
The aim of this study was to determine whether evidence could be obtained of foetal infection with Leptospira interrogans serovar hardjo in aborted foetuses collected from dairy farms. Material from 197 abortions occurring over a wide area of Victoria was collected over 3 years. None of 195 foetal kidney cultures or 7 cultures from membranes was positive for leptospiral organisms. Immunogold silver staining for leptospires was performed on sections of kidneys, lungs or heart from 156 foetuses, with negative results. Evidence of transient leptospiral infection in 11 of 123 foetuses was obtained by foetal heart blood serology. Two isolates of L. interrogans serovar hardjo were obtained from the urine of milking cows. These strains were examined by restriction endonuclease analysis and both were shown to be of the genotype Hardjobovis, as have been all Australian isolates studied so far. It appears that foetal infection with serovar hardjo is not associated with any substantial proportion of bovine abortions in Victoria, in contrast to the situation in Northern Ireland. The apparent absence from Victoria of the pathogenic genotype Hardjoprajitno is a possible explanation.
本研究的目的是确定从奶牛场收集的流产胎儿中是否能获得感染问号钩端螺旋体哈焦血清型的证据。在3年时间里，收集了维多利亚州广大地区发生的197例流产材料。195份胎儿肾脏培养物或7份胎膜培养物中均未发现钩端螺旋体呈阳性。对156例胎儿的肾脏、肺或心脏切片进行了钩端螺旋体免疫金银染色，结果均为阴性。通过胎儿心脏血液血清学检测，在123例胎儿中有11例获得了短暂钩端螺旋体感染的证据。从泌乳奶牛的尿液中分离出两株问号钩端螺旋体哈焦血清型菌株。通过限制性内切酶分析对这些菌株进行检测，结果表明它们均为Hardjobovis基因型，与迄今为止研究的所有澳大利亚分离株相同。与北爱尔兰的情况相反，在维多利亚州，哈焦血清型胎儿感染似乎与相当比例的牛流产无关。维多利亚州明显不存在致病性Hardjoprajitno基因型可能是一个解释。
