Ellis W A, O'Brien J J, Neill S D, Ferguson H W, Hanna J
Vet Rec. 1982 Feb 13;110(7):147-50. doi: 10.1136/vr.110.7.147.
Leptospiral infection was diagnosed in 41.6 per cent of 245 randomly selected aborted bovine fetuses and 68.9 per cent of 103 aborted fetuses from farms with abortion problems. Diagnosis was based on combined leptospiral isolates from fetal kidney and eye, and fetal serology and immunofluorescence. Fifty-six of the 58 strains of leptospira isolated belonged to the Hebdomadis serogroup and were similar to serovar hardjo while one of the remaining two strains belonged to the Icterohaemorrhagiae serogroup and the other to the Canicola serogroup. These findings indicate that serovar hardjo infection is a major factor in the aetiology of bovine abortion and that it is possible to isolate this organism from fetal kidney by the careful application of dilution culture technique.
在随机选取的245头流产牛胎儿中，41.6%被诊断为钩端螺旋体感染；在来自有流产问题农场的103头流产胎儿中，68.9%被诊断为钩端螺旋体感染。诊断基于从胎儿肾脏和眼睛分离出的钩端螺旋体、胎儿血清学和免疫荧光检测。分离出的58株钩端螺旋体中，56株属于七日热血清群，与哈焦血清型相似，其余两株中一株属于出血性黄疸型血清群，另一株属于犬型血清群。这些发现表明，哈焦血清型感染是牛流产病因的一个主要因素，并且通过仔细应用稀释培养技术，有可能从胎儿肾脏中分离出这种病原体。
