Ermakova Inna T, Shushkova Tatyana V, Sviridov Alexey V, Zelenkova Nina F, Vinokurova Natalya G, Baskunov Boris P, Leontievsky Alexey A
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 5 Prospect Nauki, Pushchino, Moscow, 142290, Russia.
Arch Microbiol. 2017 Jul;199(5):665-675. doi: 10.1007/s00203-017-1343-8. Epub 2017 Feb 9.
Four bacterial strains from glyphosate- or alkylphosphonates-contaminated soils were tested for ability to utilize different organophosphonates. All studied strains readily utilized methylphosphonic acid and a number of other phosphonates, but differed in their ability to degrade glyphosate. Only strains Ochrobactrum anthropi GPK 3 and Achromobacter sp. Kg 16 utilized this compound after isolation from enrichment cultures with glyphosate. Achromobacter sp. MPK 7 from the same enrichment culture, similar to Achromobacter sp. MPS 12 from methylphosphonate-polluted source, required adaptation to growth on GP. Studied strains varied significantly in their growth parameters, efficiency of phosphonates degradation and characteristic products of this process, as well as in their energy metabolism. These differences give grounds to propose a possible model of interaction between these strains in microbial consortium in phosphonate-contaminated soils.
对从草甘膦或烷基膦酸盐污染土壤中分离出的四株细菌菌株进行了测试，以检测它们利用不同有机膦酸盐的能力。所有研究的菌株都能轻易利用甲基膦酸和许多其他膦酸盐，但在降解草甘膦的能力上有所不同。只有嗜人苍白杆菌GPK 3和无色杆菌属Kg 16菌株在从含草甘膦的富集培养物中分离后能够利用该化合物。来自同一富集培养物的无色杆菌属MPK 7菌株，与来自甲基膦酸盐污染源的无色杆菌属MPS 12菌株相似，需要适应在草甘膦上生长。研究的菌株在生长参数、膦酸盐降解效率和该过程的特征产物以及能量代谢方面存在显著差异。这些差异为提出膦酸盐污染土壤中微生物群落中这些菌株之间相互作用的可能模型提供了依据。
