Liu Yang, Zhang Yu, Qian Kun, Zhang Lin, Yu Tian
Department of Anesthesiology, Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.
Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi, 563000, China.
Neurochem Res. 2017 May;42(5):1387-1393. doi: 10.1007/s11064-017-2187-y. Epub 2017 Feb 9.
The ventrolateral preoptic nucleus is a sleep-promoting nucleus located in the basal forebrain. A commonly used intravenous anesthetic, propofol, had been reported to induce sleep spindles and augment the firing rate of neurons in ventrolateral preoptic nucleus, but the underlining mechanism is yet to be known. By using patch clamp recording on neuron in acute brain slice, present study tested if histaminergic H1 and H2 receptors play a role in the effect of propofol on the noradrenalin-inhibited neurons in ventrolateral preoptic nucleus. We found that the firing rate of noradrenalin-inhibited neurons were significantly augmented by propofol; the frequency of inhibitory postsynaptic currents of noradrenalin-inhibited neuron were evidently attenuated by propofol; such inhibition effect was suppressed by histamine; and both triprolidine (antagonist for H1 histamine receptor) and ranitidine (antagonist for H2 histamine receptor) were able to increase the inhibition rate of propofol in presence of histamine. Present study demonstrated that propofol-induced inhibition of inhibitory postsynaptic currents on noradrenalin-inhibited neurons were mediated by histaminergic H1 and H2 receptors.
腹外侧视前核是位于基底前脑的一个促进睡眠的核团。一种常用的静脉麻醉药丙泊酚，据报道可诱导睡眠纺锤波并提高腹外侧视前核中神经元的放电率，但其潜在机制尚不清楚。通过在急性脑片中对神经元进行膜片钳记录，本研究测试了组胺能H1和H2受体在丙泊酚对腹外侧视前核中去甲肾上腺素抑制神经元的作用中是否发挥作用。我们发现，丙泊酚显著提高了去甲肾上腺素抑制神经元的放电率；丙泊酚明显减弱了去甲肾上腺素抑制神经元的抑制性突触后电流频率；组胺可抑制这种抑制作用；并且在存在组胺的情况下，曲普利啶（组胺H1受体拮抗剂）和雷尼替丁（组胺H2受体拮抗剂）均能够提高丙泊酚的抑制率。本研究表明，丙泊酚对去甲肾上腺素抑制神经元的抑制性突触后电流的诱导作用是由组胺能H1和H2受体介导的。
