Nakajima S, Komuro T, Shimamura M, Hazato T
Department of Anesthesiology, School of Medicine, Juntendo University, Tokyo, Japan.
Biochem Int. 1989 Sep;19(3):529-36.
An enkephalin-binding protein was found in human plasma and serum. The protein was partially purified by DEAE-cellulose column chromatography. The binding of [3H]leucine-enkephalin to this protein was competitively inhibited by unlabeled leucine- and methionine-enkephalin and various peptide hormones such as beta-endorphin and glucagon, but not by Leu-enkephalin-amide. The fact that amide derivatives of leucine-enkephalin and methionine-enkephalin did not inhibit the binding suggests that c-terminuses of enkephalins might have an important part in binding the protein. From these results, physiological roles of the enkephalin-binding protein are discussed.
在人血浆和血清中发现了一种脑啡肽结合蛋白。该蛋白通过DEAE - 纤维素柱色谱法进行了部分纯化。[3H]亮氨酸 - 脑啡肽与该蛋白的结合受到未标记的亮氨酸 - 和甲硫氨酸 - 脑啡肽以及各种肽类激素（如β - 内啡肽和胰高血糖素）的竞争性抑制，但不受亮氨酸 - 脑啡肽酰胺的抑制。亮氨酸 - 脑啡肽和甲硫氨酸 - 脑啡肽的酰胺衍生物不抑制结合这一事实表明，脑啡肽的C末端可能在与该蛋白的结合中起重要作用。根据这些结果，讨论了脑啡肽结合蛋白的生理作用。
