Dang Irène, Linkner Joern, Yan Jun, Irimia Daniel, Faix Jan, Gautreau Alexis
Ecole Polytechnique, Université Paris-Saclay, CNRS UMR7654, Palaiseau, 91120, France.
Institute for Biophysical Chemistry, Hannover Medical School, Hannover, 30623, Germany.
Biol Cell. 2017 Apr;109(4):162-166. doi: 10.1111/boc.201600064. Epub 2017 Mar 7.
Arpin is an Arp2/3 inhibitory protein, which decreases the protrusion lifetime and hence directional persistence in the migration of diverse cells. Arpin is activated by the small GTPase Rac, which controls cell protrusion, thus closing a negative feedback loop that renders the protrusion intrinsically unstable. Because of these properties, it was proposed that Arpin might play a role in directed migration, where directional persistence has to be fine-tuned. We report here, however, that Arpin-depleted tumour cells and Arpin knock-out Dictyostelium amoeba display no obvious defect in chemotaxis. These results do not rule out a potential role of Arpin in other systems, but argue against a general role of Arpin in chemotaxis.
Arpin是一种Arp2/3抑制蛋白，它会缩短突起寿命，从而降低多种细胞迁移时的定向持续性。Arpin由小GTP酶Rac激活，Rac控制细胞突起，从而形成一个负反馈回路，使突起本质上不稳定。由于这些特性，有人提出Arpin可能在定向迁移中发挥作用，在定向迁移中，定向持续性必须进行微调。然而，我们在此报告，Arpin缺失的肿瘤细胞和Arpin基因敲除的盘基网柄菌变形虫在趋化性方面没有明显缺陷。这些结果并不排除Arpin在其他系统中的潜在作用，但反对Arpin在趋化性中具有普遍作用的观点。
