National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Nanotechnology Research Center & Department of Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155-6451, Iran.
Nanomedicine (Lond). 2017 Mar;12(6):597-613. doi: 10.2217/nnm-2016-0302. Epub 2017 Feb 10.
To develop a novel anti-CD47 single-chain variable fragment (scFv) functionalized magnetic nanoparticles (MNPs) for targeting bladder cell lines and its applicability in thermotherapy.
MATERIAL & METHODS: An immunized murine antibody phage display library was constructed and screened to isolate anti-CD47 binders. A scFv was selected and conjugated to MNPs which was then utilized to discriminate CD47 bladder cells along with assessing its efficacy in thermotherapy.
An scFv with high affinity to bladder cells was efficiently conjugated to MNPs. Following a hyperthermia treatment, the function of scFv-MNP conjugates led to a considerable reduction in cell viability.
The anti-CD47 scFv-MNP conjugate was an effective cancer cell thermotherapy tool that might pave the way for development of bionano-based targeting techniques in both early detection and treatment of cancer.
开发一种新型抗 CD47 单链可变片段（scFv）功能化磁性纳米颗粒（MNPs），用于靶向膀胱细胞系及其在热疗中的应用。
构建并筛选免疫小鼠抗体噬菌体展示文库，以分离抗 CD47 结合物。选择 scFv 并将其连接到 MNPs 上，然后利用它来区分 CD47 膀胱细胞，并评估其在热疗中的疗效。
高效地将对膀胱细胞具有高亲和力的 scFv 连接到 MNPs 上。在热疗处理后，scFv-MNP 缀合物的功能导致细胞活力显著降低。
抗 CD47 scFv-MNP 缀合物是一种有效的癌症细胞热疗工具，可能为基于生物纳米的癌症早期检测和治疗靶向技术的发展铺平道路。
