Diler Ebru, Unver Turgay, Karakülah Gökhan
J Integr Bioinform. 2016 Dec 31;13(5):308. doi: 10.2390/biecoll-jib-2016-308.
Hyperhydricity is a syndrome that causes morpho-physiological malformations in tissue culture plantlets. Micro-RNAs (miRNA) are small non-coding RNAs that play important regulatory roles in plant development, stress response, and adaptation to environmental conditions. In this study, differential expression analysis indicated that miRNAs play an underlying role in the responses to the hyperhydricity syndrome in peach Prunus persica (L.) leaves. 24 known and three novel potential miRNAs were characterized in hyperhydric and non-hyperhydric transcriptome libraries. The miRNA-target transcript analyses indicated that transport, plant cuticle development, intracellular part, and stress response are regulated by miRNAs in hyperhydric leaves. It is also suggested that miR5021 and miRnovel2 might play critical regulatory roles in hyperhydricity regarding miRNA-based response to stress. This study went one step further to advance understanding of molecular miRNA-based regulatory mechanisms regarding responses to hyperhydricity in peach.
玻璃化是一种在组织培养苗中导致形态生理畸形的综合征。微小RNA（miRNA）是一类小的非编码RNA，在植物发育、应激反应和适应环境条件方面发挥着重要的调控作用。在本研究中，差异表达分析表明，miRNA在桃（Prunus persica (L.)）叶片对玻璃化综合征的反应中起着潜在作用。在玻璃化和非玻璃化转录组文库中鉴定出了24个已知的和3个新的潜在miRNA。miRNA-靶转录本分析表明，玻璃化叶片中的miRNA对运输、植物角质层发育、细胞内部分和应激反应具有调控作用。研究还表明，就基于miRNA的应激反应而言，miR5021和miRnovel2可能在玻璃化中发挥关键调控作用。本研究进一步推进了对桃中基于miRNA的玻璃化反应分子调控机制的理解。
