Martino N A, Marzano G, Mangiacotti M, Miedico O, Sardanelli A M, Gnoni A, Lacalandra G M, Chiaravalle A E, Ciani E, Bogliolo L, Minervini F, Pizzi F, Dell'Aquila M E
Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Via Manfredonia 20, 71121 Foggia, Italy; Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy.
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy.
Reprod Toxicol. 2017 Apr;69:132-145. doi: 10.1016/j.reprotox.2017.02.005. Epub 2017 Feb 7.
Cadmium is a highly toxic heavy metal with negative effects on oocyte fertilization. The aim of this study was to analyse whether cadmium-induced impairment of fertilization is caused by mitochondria dysfunction and oxidative stress in the cumulus-oocyte complex (COC). Preliminarily, 19 trace element levels were measured in ovaries from juvenile and adult ewes and age-related cadmium ovarian bioaccumulation at nanomolar concentrations was found. COCs from juvenile and adult ewes, exposed during in vitro maturation to 1nM or 100nM CdCl, and subjected to in vitro fertilization showed significantly lower fertilization rates in exposed COCs compared with controls. In vitro matured exposed and control COCs underwent confocal microscopy analysis of mitochondria activity and reactive oxygen species (ROS) levels and lipid peroxidation (LPO) assay at cumulus cell and oocyte level. In both age groups, cadmium at nanomolar concentrations induced cumulus-oocyte mitochondria over-activity and oxidative damage which were related to impaired oocyte fertilization.
镉是一种剧毒重金属，对卵母细胞受精有负面影响。本研究的目的是分析镉诱导的受精障碍是否由卵丘-卵母细胞复合体（COC）中的线粒体功能障碍和氧化应激引起。初步研究中，测量了幼年和成年母羊卵巢中的19种微量元素水平，发现了与年龄相关的纳摩尔浓度镉在卵巢中的生物蓄积。来自幼年和成年母羊的COC在体外成熟过程中暴露于1nM或100nM CdCl，并进行体外受精，结果显示，与对照组相比，暴露的COC受精率显著降低。对体外成熟的暴露组和对照组COC进行共聚焦显微镜分析，以检测线粒体活性、活性氧（ROS）水平，并在卵丘细胞和卵母细胞水平进行脂质过氧化（LPO）测定。在两个年龄组中，纳摩尔浓度的镉均诱导了卵丘-卵母细胞线粒体过度活跃和氧化损伤，这与卵母细胞受精受损有关。
