Figueredo C M, Martins A P, Lira-Junior R, Menegat J B, Carvalho A T, Fischer R G, Gustafsson A
Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Department of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Cytokine. 2017 Jul;95:1-6. doi: 10.1016/j.cyto.2017.01.016. Epub 2017 Feb 9.
This study assessed the cytokine expression in gingival and intestinal tissues from periodontitis patients with inflammatory bowel disease (IBD) and evaluated if IBD activity is a covariate to the amount of gingival cytokines. Paired gingival and intestinal tissues were collected from 21 patients and homogenised using a cell disruptor. Cytokine expression (IL-1β, IL-4, IL-6, IL-10, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IL-17A, IL-17F, IFN-γ, sCD40L, and TNF-α) was evaluated using bead-based multiplex technology. An inflammation score was developed using the intestinal cytokines that showed good accuracy to discriminate IBD active patients from those in remission and then a similar score was applied to gingival tissue. IL-4, IL-10 and IL-21 expressions were significantly increased in gingival tissue from patients with an active disease as compared to those with a disease in remission. The inflammation score (mean value of IL-1β, IL-6, IL-21, and sCD40L) was significantly higher in gingival tissue from patients with IBD activity. There was a significant correlation between gingival and intestinal inflammation scores (rho=0.548; P=0.01). Significantly higher IL-23 and IFN-γ levels and lower IL-31 and TNF-α levels were observed in gingival tissues than in intestinal ones. Activity of inflammatory bowel disease influenced the cytokine expression in gingival tissue.
本研究评估了炎症性肠病（IBD）牙周炎患者牙龈组织和肠道组织中的细胞因子表达，并评估IBD活动是否是牙龈细胞因子数量的协变量。从21例患者中采集配对的牙龈组织和肠道组织，并用细胞破碎仪进行匀浆。使用基于微珠的多重技术评估细胞因子表达（IL-1β、IL-4、IL-6、IL-10、IL-21、IL-22、IL-23、IL-25、IL-31、IL-33、IL-17A、IL-17F、IFN-γ、sCD40L和TNF-α）。利用肠道细胞因子制定了炎症评分，该评分在区分IBD活动期患者和缓解期患者方面具有良好的准确性，然后将类似的评分应用于牙龈组织。与疾病缓解期患者相比，疾病活动期患者牙龈组织中IL-4、IL-10和IL-21的表达显著增加。IBD活动期患者牙龈组织中的炎症评分（IL-1β、IL-6、IL-21和sCD40L的平均值）显著更高。牙龈炎症评分与肠道炎症评分之间存在显著相关性（rho=0.548；P=0.01）。观察到牙龈组织中的IL-23和IFN-γ水平显著高于肠道组织，而IL-31和TNF-α水平则低于肠道组织。炎症性肠病的活动影响牙龈组织中的细胞因子表达。
