Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, Santiago, Chile; Department of Conservative Dentistry, Faculty of Dentistry, Universidad de Chile, Santiago, Chile.
Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, Santiago, Chile.
Arch Oral Biol. 2017 Jun;78:26-33. doi: 10.1016/j.archoralbio.2017.02.008. Epub 2017 Feb 6.
In Aggregatibacter actinomycetemcomitans, different serotypes have been described based on lipopolysaccharide (LPS) antigenicity. When T lymphocytes were stimulated with these serotypes, different patterns of T-helper (Th)1 and Th17-type of immune responses were reported. Recently, two new Th phenotypes have been described and named Th9 and Th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. This study aimed to investigate the potential Th9 and/or Th22 lymphocyte responses when stimulated with autologous dendritic cells infected with different A. actinomycetemcomitans serotypes.
Monocyte-derived dendritic cells and naïve CD4 T lymphocytes were obtained from healthy donors and stimulated with different serotypes of A. actinomycetemcomitans at a multiplicity of infection MOI=10 or their purified LPS (10-50ng/ml). The levels for the Th9 and Th22-associated cytokines, as well as the transcription factor master-switch genes implied in their differentiation Spi-B and AhR, were quantified by qPCR and ELISA.
When stimulated with the serotype b of A. actinomycetemcomitans, higher levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected in dendritic cells, as well as higher levels of IL-22 and AhR were detected in T lymphocytes, when compared with stimulation with the other serotypes.
The serotype b of A. actinomycetemcomitans has a higher capacity of trigger Th22-type of immune response in both dendritic cells and T lymphocytes. These data allow us to suggest that, when the serotype b of A. actinomycetemcomitans is a significant part of the subgingival biofilm, the Th22 polarization might be triggered within the periodontal lesion.
在伴放线放线杆菌中，根据脂多糖（LPS）抗原性已描述了不同的血清型。当 T 淋巴细胞受到这些血清型的刺激时，报告了不同模式的 T 辅助（Th）1 和 Th17 型免疫反应。最近，已经描述了两种新的 Th 表型，并将其命名为 Th9 和 Th22 淋巴细胞；然而，它们在牙周炎发病机制中的作用尚不清楚。本研究旨在研究用自体感染不同伴放线放线杆菌血清型的树突状细胞刺激时潜在的 Th9 和/或 Th22 淋巴细胞反应。
从健康供体中获得单核细胞衍生的树突状细胞和幼稚 CD4 T 淋巴细胞，并以感染复数（MOI）=10 或其纯化 LPS（10-50ng/ml）刺激不同血清型的伴放线放线杆菌。通过 qPCR 和 ELISA 定量 Th9 和 Th22 相关细胞因子以及分化所需的转录因子主开关基因 Spi-B 和 AhR 的水平。
与刺激其他血清型相比，当用伴放线放线杆菌血清型 b 刺激时，树突状细胞中检测到更高水平的白细胞介素（IL）-6 和肿瘤坏死因子（TNF）-α，T 淋巴细胞中检测到更高水平的 IL-22 和 AhR。
伴放线放线杆菌血清型 b 具有在树突状细胞和 T 淋巴细胞中引发 Th22 型免疫反应的更高能力。这些数据使我们能够提出这样的假设，即当伴放线放线杆菌的血清型 b 是龈下生物膜的重要组成部分时，Th22 极化可能在牙周病变内被触发。
