School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China; School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.
School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China.
Bioresour Technol. 2017 May;231:19-25. doi: 10.1016/j.biortech.2017.01.038. Epub 2017 Jan 25.
A strain, Brevibacillus agri DH-1, isolated from dry lands was used to remove m-dichlorobenzene. After 48h culturing, the concentrations of m-dichlorobenzene decreased from 26-130 to 7.87-28.87mg/L and dry cell weight for bacterial growth reached 52.43-75.05mg/L. The growth and degradation kinetics were analyzed by the fitting of Haldane-Andrews model and pseudo first-order model. A degradation pathway was proposed according to major intermediates (phenol), chloride ion variation, ring-opening enzyme activity, and high mineralization (0.47g/g, 0.65 g/g, 0.15 g/g). In addition, the performance in a biotrickling filter (BTF) was evaluated through removal efficiency and pressure drop values with increasing inlet loading rate from 4.10 to 122.57g/m/h at three empty bed residence time points (30s, 60s, and 90s). The results demonstrated that strain DH-1 possessed high removal efficiency and stable operation in a BTF.
从旱地中分离出一株短小芽孢杆菌 DH-1，用于去除间二氯苯。经过 48 小时培养，间二氯苯的浓度从 26-130 降低到 7.87-28.87mg/L，细菌生长的干重达到 52.43-75.05mg/L。通过拟合 Haldane-Andrews 模型和伪一级模型对生长和降解动力学进行了分析。根据主要中间体（苯酚）、氯离子变化、开环酶活性和高矿化度（0.47g/g、0.65g/g、0.15g/g）提出了降解途径。此外，还通过去除效率和压降值评估了在生物滴滤器（BTF）中的性能，入口负载率从 4.10 增加到 122.57g/m/h，在三个空床停留时间点（30s、60s 和 90s）。结果表明，DH-1 菌株在 BTF 中具有高去除效率和稳定的运行性能。
