生物滴滤塔去除气相 1,2-二氯苯的性能评价：鼠李糖脂和铁离子的影响。
Performance evaluation of a biotrickling filter for the removal of gas-phase 1,2-dichlorobenzene: Influence of rhamnolipid and ferric ions.
机构信息
School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu, 221116, PR China.
School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu, 221116, PR China; School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, PR China.
出版信息
Chemosphere. 2020 Jul;250:126261. doi: 10.1016/j.chemosphere.2020.126261. Epub 2020 Feb 19.
The aim of this study was to evaluate the influence of rhamnolipid (RL) and ferric ions on the performance of a biotrickling filter (BTF) for the removal of gas-phase 1,2-dichlorobenzene (o-DCB). A comprehensive investigation of microbial growth, pollutant solubility, extracellular polymeric substances (EPS) and enzymatic activity in o-DCB degradation by an isolated strain Bacillus cereus DL-1 with/without RL and Fe were carried out using batch microcosm experiments. In addition, o-DCB removal performance, biofilm morphology, and microbial community structures in two identical lab-scale biotrickling filters (named BTF1 and BTF2) inoculated with strain DL-1 were studied. The batch microcosm experiments demonstrated that 120 mg L RL and 4 mg L Fe could enhance the biodegradation of o-DCB, which may be due to promotion on bacterial growth, o-DCB solubilization, CO enzyme activity, and polysaccharide (PS) and protein (PN) in EPS. Fourier transform infrared (FTIR) spectra indicated that the addition of RL with Fe had notable effects on the functional groups of PS and PN in EPS. The experimental results in BTFs indicate that the removal efficiency of o-DCB decreased from 100% to 56.4% for BTF1, which was not fed with RL and Fe, and from 100% to 80.3% for BTF2, which was fed with RL and Fe, when the inlet loading rate increased from 4.88 to 102 g m h at an empty bed residence time of 60 s. In addition, the microbial adhesive strength and the microbial community structure were different among both BTFs, highlighting the positive effects of RL and Fe.
本研究旨在评估鼠李糖脂（RL）和铁离子对生物滴滤塔（BTF）去除气相 1,2-二氯苯（o-DCB）性能的影响。通过分批微宇宙实验，对分离出的蜡状芽孢杆菌 DL-1 单独使用 RL 和 Fe 以及同时使用 RL 和 Fe 降解 o-DCB 时微生物生长、污染物溶解度、胞外聚合物（EPS）和酶活性进行了全面研究。此外，还研究了接种 DL-1 菌株的两个相同实验室规模的生物滴滤塔（分别命名为 BTF1 和 BTF2）中 o-DCB 去除性能、生物膜形态和微生物群落结构。分批微宇宙实验表明，120 mg/L RL 和 4 mg/L Fe 可以增强 o-DCB 的生物降解，这可能是由于促进了细菌生长、o-DCB 增溶、CO 酶活性以及 EPS 中的多糖（PS）和蛋白质（PN）。傅里叶变换红外（FTIR）光谱表明，RL 与 Fe 的添加对 EPS 中 PS 和 PN 的官能团有显著影响。BTFs 的实验结果表明，当入口负荷率从 4.88 g m h 增加到 102 g m h 时，BTF1（未添加 RL 和 Fe）的 o-DCB 去除效率从 100%降低到 56.4%，而 BTF2（添加 RL 和 Fe）的 o-DCB 去除效率从 100%降低到 80.3%，空床停留时间为 60 s。此外，两 BTF 中的微生物附着强度和微生物群落结构也存在差异，这突出了 RL 和 Fe 的积极作用。
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