Zhan Yali, Wang Qinghong, Chen Chunmao, Kim Jung Bong, Zhang Hongdan, Yoza Brandon A, Li Qing X
State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, 102249, China.
Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA.
J Ind Microbiol Biotechnol. 2017 Jun;44(6):845-855. doi: 10.1007/s10295-017-1909-0. Epub 2017 Feb 11.
Microbial enhanced oil recovery (MEOR) is an emerging oil extraction technology that utilizes microorganisms to facilitate recovery of crude oil in depleted petroleum reservoirs. In the present study, effects of wheat bran utilization were investigated on stimulation of indigenous MEOR. Biostimulation conditions were optimized with the response surface methodology. The co-application of wheat bran with KNO and NHHPO significantly promoted indigenous MEOR (IMEOR) and exhibited sequential aerobic (O-), facultative (A-) and anaerobic (A-) metabolic stages. The surface tension of fermented broth decreased by approximately 35%, and the crude oil was highly emulsified. Microbial community structure varied largely among and in different IMEOR metabolic stages. Pseudomonas sp., Citrobacter sp., and uncultured Burkholderia sp. dominated the O-, A- and early A-stages. Bacillus sp., Achromobacter sp., Rhizobiales sp., Alcaligenes sp. and Clostridium sp. dominated the later A-stage. This study illustrated occurrences of microbial community succession driven by wheat bran stimulation and its industrial potential.
微生物强化采油（MEOR）是一种新兴的采油技术，它利用微生物来促进在枯竭的石油储层中原油的开采。在本研究中，研究了麦麸利用对本地微生物强化采油的刺激作用。采用响应面法对生物刺激条件进行了优化。麦麸与硝酸钾和磷酸二氢铵的共同应用显著促进了本地微生物强化采油（IMEOR），并呈现出好氧（O-）、兼性（A-）和厌氧（A-）的连续代谢阶段。发酵液的表面张力降低了约35%，原油被高度乳化。微生物群落结构在不同的IMEOR代谢阶段之间和内部有很大差异。假单胞菌属、柠檬酸杆菌属和未培养的伯克霍尔德菌属在O-、A-和早期A阶段占主导地位。芽孢杆菌属、无色杆菌属、根瘤菌目、产碱菌属和梭菌属在后期A阶段占主导地位。本研究阐明了由麦麸刺激驱动的微生物群落演替的发生及其工业潜力。
