Suppr超能文献

Cellular influx of sulfobromophthalein by the biliary epithelium carcinoma cell line Sk-Cha-1 reveals kinetic criteria of a carrier-mediated uptake mechanism.

作者信息

Stremmel W, Falbrede J, Diede H E, Elsing C

机构信息

Department of Internal Medicine, Heinrich Heine University, Düsseldorf, F.R.G.

出版信息

Biochim Biophys Acta. 1989 Nov 20;1014(2):108-11. doi: 10.1016/0167-4889(89)90021-9.

DOI:10.1016/0167-4889(89)90021-9
PMID:2819084
Abstract

Cellular uptake of the cholephilic organic anion sulphobromophthalein (BSP) by the human biliary epithelium carcinoma cell line Sk-Cha-1 was examined at 37 degrees C. In confluent monolayer cultures the cellular influx rate of increasing concentrations of [35S]BSP followed saturation kinetics with a Km value of 18 microM and a Vmax value of 243 pmol.min-1.mg protein-1. Uptake of [35S]BSP was competitively inhibited by the presence of bilirubin diglucuronide, but not by taurocholate or cholate. Furthermore, uptake was temperature dependent with maximal cellular influx rates at 37 degrees C.

摘要

相似文献

1
Cellular influx of sulfobromophthalein by the biliary epithelium carcinoma cell line Sk-Cha-1 reveals kinetic criteria of a carrier-mediated uptake mechanism.
Biochim Biophys Acta. 1989 Nov 20;1014(2):108-11. doi: 10.1016/0167-4889(89)90021-9.
2
Cellular uptake of conjugated bilirubin and sulfobromophthalein (BSP) by the human hepatoma cell line Hep G2 is mediated by a membrane BSP/bilirubin binding protein.
J Hepatol. 1990 Jan;10(1):99-104. doi: 10.1016/0168-8278(90)90079-7.
3
Hepatocellular uptake of sulfobromophthalein and bilirubin is selectively inhibited by an antibody to the liver plasma membrane sulfobromophthalein/bilirubin binding protein.肝细胞膜磺溴酞钠/胆红素结合蛋白的抗体可选择性抑制肝细胞对磺溴酞钠和胆红素的摄取。
J Clin Invest. 1986 Sep;78(3):822-6. doi: 10.1172/JCI112646.
4
Bilitranslocase and sulfobromophthalein/bilirubin-binding protein are both involved in the hepatic uptake of organic anions.胆红素转运酶和磺溴酞钠/胆红素结合蛋白均参与肝脏对有机阴离子的摄取。
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8136-9. doi: 10.1073/pnas.90.17.8136.
5
Transport of sulfobromophthalein and taurocholate in the HepG2 cell line in relation to the expression of membrane carrier proteins.磺溴酞钠和牛磺胆酸盐在HepG2细胞系中的转运与膜载体蛋白表达的关系
Biochem Biophys Res Commun. 1992 Mar 31;183(3):1203-8. doi: 10.1016/s0006-291x(05)80318-3.
6
The kinetics of sulfobromophthalein uptake by rat liver sinusoidal vesicles.大鼠肝窦状隙小泡摄取磺溴酞的动力学
Biochim Biophys Acta. 1987 Apr 9;898(2):159-71. doi: 10.1016/0005-2736(87)90034-4.
7
Expression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes.鳐鱼肝脏中钠非依赖性有机阴离子摄取系统在非洲爪蟾卵母细胞中的表达。
Am J Physiol. 1995 Jan;268(1 Pt 1):G18-23. doi: 10.1152/ajpgi.1995.268.1.G18.
8
Organic anion transport in HepG2 cells: absence of the high-affinity, chloride-dependent transporter.HepG2细胞中的有机阴离子转运：缺乏高亲和力、氯离子依赖性转运体。
Hepatology. 1991 Dec;14(6):1217-23.
9
Difference in hepatic uptake of tetra- and di-bromosulfophthalein in rat. Role of hydrophobicity, binding to plasma proteins and affinity for plasma membrane carrier protein.大鼠肝脏对四溴和二溴磺酞的摄取差异。疏水性、与血浆蛋白结合及对质膜载体蛋白亲和力的作用。
Biochem Pharmacol. 1993 Sep 1;46(5):925-31. doi: 10.1016/0006-2952(93)90503-o.
10
Role of BSP/bilirubin binding protein and bilitranslocase in glutathione uptake in rat basolateral liver plasma membrane vesicles.骨桥蛋白/胆红素结合蛋白和胆转运蛋白在大鼠肝基底外侧质膜囊泡谷胱甘肽摄取中的作用
Biochem Biophys Res Commun. 1994 Apr 29;200(2):1079-85. doi: 10.1006/bbrc.1994.1560.

引用本文的文献

1
Comparison of the disposition of hepatically-generated morphine-3-glucuronide and morphine-6-glucuronide in isolated perfused liver from the guinea pig.豚鼠离体灌注肝脏中肝源性吗啡-3-葡萄糖醛酸苷和吗啡-6-葡萄糖醛酸苷处置情况的比较。
Pharm Res. 1997 Aug;14(8):1014-8. doi: 10.1023/a:1012145126847.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验