肝细胞膜磺溴酞钠/胆红素结合蛋白的抗体可选择性抑制肝细胞对磺溴酞钠和胆红素的摄取。
Hepatocellular uptake of sulfobromophthalein and bilirubin is selectively inhibited by an antibody to the liver plasma membrane sulfobromophthalein/bilirubin binding protein.
作者信息
Stremmel W, Berk P D
出版信息
J Clin Invest. 1986 Sep;78(3):822-6. doi: 10.1172/JCI112646.
Abstract
To clarify sulfobromophthalein (BSP) and bilirubin uptake mechanisms, isolated rat hepatocytes were incubated with [35S]BSP. The initial uptake velocity (V0), determined from the first, linear portion of the cumulative uptake curve, was saturable (Michaelis constant [Km] = 6.2 +/- 0.5 microM; Vmax = 638 +/- 33 pmol X min-1 per 10(5) hepatocytes), maximal at 37 degrees C and pH 7.4, and competitively inhibited by bilirubin, but not by taurocholate, cholate, or oleate. Preloading with unlabeled BSP led to trans-stimulation of V0. Sodium substitution or pretreatment of hepatocytes with ouabain or metabolic inhibitors had no effect on V0; trypsin reduced V0 by 39% (P less than 0.001). A rabbit antiserum to the rat liver plasma membrane (LPM)-BSP/bilirubin binding protein selectively reduced V0 of 5 microM [35S]BSP and [14C]bilirubin by 41 and 42%, respectively (P less than 0.01); uptakes of [3H]oleate, [3H]cholate and [3H]taurocholate were not affected. Hence, the LPM-BSP/bilirubin binding protein plays a role in the carrier-mediated uptake of BSP and bilirubin by hepatocytes.
摘要
为阐明磺溴酞钠（BSP）和胆红素的摄取机制，将分离的大鼠肝细胞与[35S]BSP一起孵育。根据累积摄取曲线的第一个线性部分确定的初始摄取速度（V0）是可饱和的（米氏常数[Km]=6.2±0.5微摩尔；Vmax=638±33皮摩尔·分钟-1/10(5)个肝细胞），在37℃和pH7.4时最大，且受到胆红素的竞争性抑制，但不受牛磺胆酸盐、胆酸盐或油酸盐的抑制。用未标记的BSP预加载导致V0的反式刺激。用钠替代或用哇巴因或代谢抑制剂预处理肝细胞对V0没有影响；胰蛋白酶使V0降低39%（P<0.001）。一种针对大鼠肝细胞膜（LPM）-BSP/胆红素结合蛋白的兔抗血清分别使5微摩尔[35S]BSP和[14C]胆红素的V0选择性降低41%和42%（P<0.01）；[3H]油酸盐、[3H]胆酸盐和[3H]牛磺胆酸盐的摄取不受影响。因此，LPM-BSP/胆红素结合蛋白在肝细胞对BSP和胆红素的载体介导摄取中起作用。
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本文引用的文献
1
Some formal approaches to the analysis of kinetic data in terms of linear compartmental systems.一些根据线性房室系统对动力学数据进行分析的形式化方法。
Biophys J. 1962 May;2(3):289-316. doi: 10.1016/s0006-3495(62)86856-8.
2
The routine fitting of kinetic data to models: a mathematical formalism for digital computers.动力学数据与模型的常规拟合：一种适用于数字计算机的数学形式体系。
Biophys J. 1962 May;2(3):275-87. doi: 10.1016/s0006-3495(62)86855-6.
3
Identification, purification, and partial characterization of an organic anion binding protein from rat liver cell plasma membrane.大鼠肝细胞质膜中一种有机阴离子结合蛋白的鉴定、纯化及部分特性分析。
J Clin Invest. 1980 May;65(5):1152-61. doi: 10.1172/JCI109770.
4
Characterization of the anion transport system in hepatocyte plasma membranes.肝细胞质膜中阴离子转运系统的特性分析
J Biol Chem. 1980 Apr 10;255(7):2637-40.
5
Albumin helps mediate removal of taurocholate by rat liver.白蛋白有助于介导大鼠肝脏对牛磺胆酸盐的清除。
J Clin Invest. 1981 May;67(5):1517-22. doi: 10.1172/jci110182.
6
Binding of unconjugated and conjugated sulfobromophthalein to rat liver plasma membrane fractions in vitro.未结合和结合的磺溴酞在体外与大鼠肝细胞膜组分的结合。
Biochim Biophys Acta. 1981 Jan 8;640(1):298-312. doi: 10.1016/0005-2736(81)90554-x.
7
Effect of albumin binding on the hepatic transport of rose bengal: surface-mediated dissociation of limited capacity.白蛋白结合对孟加拉玫瑰红肝脏转运的影响：表面介导的有限容量解离
J Pharmacol Exp Ther. 1982 Nov;223(2):342-7.
8
Transport of sodium, chloride, and taurocholate by cultured rat hepatocytes.培养的大鼠肝细胞对钠、氯和牛磺胆酸盐的转运。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):986-90. doi: 10.1073/pnas.78.2.986.
9
Reconstitution in vitro of sulfobromophthalein transport by bilitranslocase.通过胆转运蛋白在体外重建磺溴酞的转运。
Biochim Biophys Acta. 1982 Feb 23;685(2):123-8. doi: 10.1016/0005-2736(82)90088-8.
10
Further studies on bilitranslocase, a plasma membrane protein involved in hepatic organic anion uptake.
Biochim Biophys Acta. 1982 Feb 23;685(2):117-22. doi: 10.1016/0005-2736(82)90087-6.
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