免疫组织化学技术方面的数据库。

Databases for technical aspects of immunohistochemistry.

作者信息

Furukawa Satoshi, Nagaike Mika, Ozaki Kiyokazu

机构信息

Conference on Experimental Animal Histopathology; Biological Research Laboratories, Nissan Chemical Industries, Ltd., 1470 Shiraoka, Shiraoka-shi, Saitama 349-0294, Japan.

Conference on Experimental Animal Histopathology; Regulatory Affairs Division, Biosciences Sales & Marketing, Ishihara Sangyo Kaisha, Ltd., 2-3-1, Nishishibukawa, Kusatsu, Shiga 525-0025, Japan.

出版信息

J Toxicol Pathol. 2017 Jan;30(1):79-107. doi: 10.1293/tox.2016-0047. Epub 2016 Sep 26.

DOI:10.1293/tox.2016-0047

PMID:28190929

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5293696/

Abstract

With the aims of sharing information about the technical aspects of immunohistochemistry (IHC) and making it possible to make a suitable choice of antibody for histopathological examination, this technical report describes the results of a questionnaire administered during the period of 2014 to 2015 to members of the Conference on Experimental Animal Histopathology. It also describes the immunological properties of primary antibodies (clone, supplier, catalog number, species reactivity, etc.) and the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, secondary antibody information, etc.) for a total number of 733 primary antibodies (425 kinds of primary antibody).

摘要

为了分享免疫组织化学（IHC）技术方面的信息，并使人们能够为组织病理学检查选择合适的抗体，本技术报告描述了在2014年至2015年期间对实验动物组织病理学会议成员进行问卷调查的结果。它还描述了总共733种一抗（425种不同的一抗）的免疫学特性（克隆、供应商、目录编号、物种反应性等）以及免疫组织化学染色条件（固定液、固定时间、包埋、抗原修复方法、抗体稀释度、孵育时间、孵育温度、阳性对照组织、二抗信息等）。