DTU Centre for Nuclear Technologies (NUTECH), Technical University of Denmark, Risø Campus, DK 4000 Roskilde, Denmark.
Methods Appl Fluoresc. 2016 Nov 14;4(4):045002. doi: 10.1088/2050-6120/4/4/045002.
Photodynamic diagnosis (PDD) of bladder tumour tissue significantly improves endoscopic diagnosis and treatment of bladder cancer in rigid cystoscopes in the operating theatre and thus reduces tumour recurrence. PDD comprises the use of blue light, which unfortunately excites green fluorescence from urine. As this green fluorescence confounds the desired red fluorescence of the PDD, methods for avoiding this situation particularly in cystoscopy using flexible cystoscopes are desirable. In this paper we demonstrate how a tailor made high power LED light source at 525 nm can be used for fluorescence assisted tumour detection using both a flexible and rigid cystoscope used in the outpatient department (OPD) and operating room (OR) respectively. It is demonstrated both in vitro and in vivo how this light source can significantly reduce the green fluorescence problem with urine. At the same time this light source also is useful for exciting autofluorescence in healthy bladder mucosa. This autofluorescence then provides a contrast to the sensitized fluorescence (PDD) of tumours in the bladder.
光动力诊断（PDD）显著提高了膀胱肿瘤组织的内镜诊断和治疗效果，从而降低了肿瘤的复发率。PDD 包括使用蓝光，但不幸的是，它会激发尿液中的绿色荧光。由于这种绿色荧光会干扰 PDD 所需的红色荧光，因此特别需要寻找在使用软性膀胱镜进行膀胱镜检查时避免这种情况的方法。在本文中，我们展示了如何使用定制的 525nm 高功率 LED 光源，分别在门诊（OPD）和手术室（OR）使用的软性和硬性膀胱镜进行荧光辅助肿瘤检测。既在体外又在体内证明了这种光源如何显著减少尿液中的绿色荧光问题。同时，这种光源也可用于激发健康膀胱黏膜的自发荧光。然后，这种自发荧光为膀胱中的肿瘤提供了敏化荧光（PDD）的对比。
