Kodama M, Kodama T, Inoue F, Kodama M
Kodama Research Institute of Preventive Medicine, Nagoya, Japan.
Biomed Pharmacother. 1989;43(7):519-26. doi: 10.1016/0753-3322(89)90114-5.
The present study has investigated comparatively the in vivo effects of physiologic saline drink, N-methylN'-nitro-n-nitrosoguanidine (MNNG) drink, saline-MNNG drink and hydrocortisone treatment in terms of 1 functional parameter (daily liquid consumption) and 3 weight parameters (weights of whole body, forestomach and glandular stomach) using Swiss/ICR mice of both sexes. The results obtained are as follows: 1) The sole use of hydrocortisone treatment significantly increased daily water consumption in mice of both sexes. 2) The promoting effect of the hormone on liquid consumption was further accelerated by concomitant use of physiologic saline. There was no sex difference in the extent of drink increase, but the figure of male mice was more variable than that of female mice. 3) The introduction of the 3rd stressor MNNG significantly reduced the salt-hydrocortisone-linked acceleration of liquid consumption. The extent of MNNG suppression was more marked in male mice than in female mice. 4) The responses of the 3 weight parameters to hydrocortisone were found to be tissue- and sex-specific. The dynamic changes of the above 4 parameters under the influence of the 3 stressors are in agreement with the interpretation that androgen plays a positive role in the salt-hydrocortisone-linked acceleration of liquid consumption, and that MNNG reverses the drink increase by inhibiting the binding of androgen with its receptor.
本研究使用雌雄兼有的瑞士/ICR小鼠，比较研究了生理盐水饮品、N-甲基-N'-硝基-N-亚硝基胍（MNNG）饮品、生理盐水-MNNG饮品及氢化可的松处理对1个功能参数（每日液体摄入量）和3个体重参数（全身、前胃和腺胃的重量）的体内影响。所得结果如下：1）单独使用氢化可的松处理显著增加了雌雄小鼠的每日饮水量。2）同时使用生理盐水进一步加速了激素对液体摄入的促进作用。饮水量增加的程度无性别差异，但雄性小鼠的数据比雌性小鼠更具变异性。3）引入第三种应激源MNNG显著降低了盐-氢化可的松相关的液体摄入加速作用。MNNG的抑制程度在雄性小鼠中比在雌性小鼠中更明显。4）发现3个体重参数对氢化可的松的反应具有组织和性别特异性。上述4个参数在3种应激源影响下的动态变化与以下解释一致：雄激素在盐-氢化可的松相关的液体摄入加速中起积极作用，而MNNG通过抑制雄激素与其受体的结合来逆转饮水量的增加。
