Institute of Macromolecular Chemistry AS CR v.v.i. , Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
Biosensor Technologies, AIT-Austrian Institute of Technology GmbH , Muthgasse 11, 1190 Vienna, Austria.
Anal Chem. 2017 Mar 7;89(5):2972-2977. doi: 10.1021/acs.analchem.6b04432. Epub 2017 Feb 21.
A biosensor for the detection of hepatitis B antibodies in clinical saliva was developed. Compared to conventional analysis of blood serum, it offers the advantage of noninvasive collection of samples. Detection of biomarkers in saliva imposes two major challenges associated with the low analyte concentration and increased surface fouling. The detection of minute amounts of hepatitis B antibodies was performed by plasmonically amplified fluorescence sandwich immunoassay. To have access to specific detection, we prevented the nonspecific adsorption of biomolecules present in saliva by brushes of poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] grafted from the gold sensor surface and post modified with hepatitis B surface antigen. Obtained results were validated against the response measured with ELISA at a certified laboratory using serum from the same patients.
开发了一种用于检测临床唾液中乙型肝炎抗体的生物传感器。与传统的血清分析相比，它具有非侵入性采集样本的优势。在唾液中检测生物标志物存在两个主要挑战，即分析物浓度低和表面污垢增加。通过等离子体放大荧光三明治免疫测定法检测极少量的乙型肝炎抗体。为了进行特异性检测，我们通过从金传感器表面接枝并随后用乙型肝炎表面抗原修饰的聚[(N-(2-羟丙基)甲基丙烯酰胺)-共-(羧基甜菜碱甲基丙烯酰胺)]刷防止了唾液中存在的生物分子的非特异性吸附。将获得的结果与使用同一患者血清在认证实验室中用 ELISA 测量的响应进行了验证。
