基于上转换荧光粉技术的侧向流动分析法用于快速定量检测乙型肝炎表面抗体的研究进展
Development of up-converting phosphor technology-based lateral-flow assay for rapidly quantitative detection of hepatitis B surface antibody.
作者信息
Li Luping, Zhou Lei, Yu Yang, Zhu Ziwen, Lin Changqing, Lu Changlong, Yang Ruifu
机构信息
Department of Immunology, China Medical University, Shenyang 110001, PR of China.
出版信息
Diagn Microbiol Infect Dis. 2009 Feb;63(2):165-72. doi: 10.1016/j.diagmicrobio.2008.10.020.
An up-converting phosphor technology-based lateral-flow (UPT-LF) assay system was developed for rapid and quantitative detection of hepatitis B surface antibody (HBsAb). To evaluate its performance, we compared it with the Abbott Axsym AUSAB (ABBOTT Diagnostics Division, Wiesbaden, Germany) assay and conventional ELISA (Wantai Biological Pharmacy Enterprise, Beijing, China) using 13 standard positive sera and 306 clinical sera. In both laboratory evaluation and clinical application, UPT-LF assay showed the best sensitivity (99.19%) and detection agreement (97.43% for the adjusted agreement) with true results. The concordance rate between UPT-LF and ELISA, as shown by correlative regression analysis, was the highest (R(2)=0.6389), whereas that between UPT-LF and AUSAB was the lowest (R(2)=0.5702). In conclusion, UPT-LF assay for quantitative detection of HBsAb is sensitive and rapid, promising this new assay a bright future.
开发了一种基于上转换磷光体技术的侧向流动（UPT-LF）检测系统，用于快速定量检测乙型肝炎表面抗体（HBsAb）。为评估其性能，我们使用13份标准阳性血清和306份临床血清，将其与雅培Axsym AUSAB（德国威斯巴登雅培诊断部）检测法和传统酶联免疫吸附测定法（中国北京万泰生物药业股份有限公司）进行了比较。在实验室评估和临床应用中，UPT-LF检测法均显示出与真实结果最佳的灵敏度（99.19%）和检测一致性（校正一致性为97.43%）。相关回归分析显示，UPT-LF与酶联免疫吸附测定法之间的符合率最高（R(2)=0.6389），而UPT-LF与AUSAB之间的符合率最低（R(2)=0.5702）。总之，用于定量检测HBsAb的UPT-LF检测法灵敏且快速，预示着这种新检测法前景光明。
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