Matuszczak Ewa, Tylicka Marzena, Dębek Wojciech, Sankiewicz Anna, Gorodkiewicz Ewa, Hermanowicz Adam
Department of Pediatric Surgery, Medical University of Bialystok, Bialystok, Poland.
Department of Biophysics, Medical University of Bialystok, Bialystok, Poland.
Adv Med Sci. 2017 Mar;62(1):83-86. doi: 10.1016/j.advms.2016.07.004. Epub 2017 Feb 11.
The study aims to determinate concentrations of ubiquitin C-terminal hydrolase 1 (UCHL1), which hydrolyzes amino acids from ubiquitin and cleave di-ubiquitins, in serum of children after thermal injury.
PATIENTS/METHODS: 42 children scalded by hot water, managed at the Department of Pediatric Surgery, with burns in 4-20% TBSA were included into the study (age 9 months up to 14 years, mean age 2.5±1 years). Blood plasma UCHL1 concentration was assessed in 2-6h, 12-16h, 3d, 5d, and 7d after injury using surface plasmon resonance imaging biosensor. 18 healthy subjects admitted for planned surgeries served as controls.
The UCHL1 concentration in the blood plasma of patients with thermal injuries reached its peak 12-16h after thermal injury and slowly decreased over time, and still did not reach the normal range on the 7th day after thermal injury. Mean concentrations of UCHL1 after thermal injury were above the range measured in controls (0.12ng/ml): 2-6h after injury - 5.59ng/dl, 12-16h after injury - 9.16ng/dl, 3 days after injury - 6.94ng/dl, 5 days after 5.41ng/dl, 7 days after injury - 4.09ng/dl.
We observed sudden increase in the concentration of UCHL1 2-16h after thermal injury with the slow decrease in the UCHL1 concentration over the time. UCHL1 concentration was proportional to the severity of the burn. Further studies are needed to determine the mechanisms by which UCHL1 contributes to metabolic response following thermal injury.
本研究旨在测定热损伤后儿童血清中泛素C末端水解酶1（UCHL1）的浓度，UCHL1可从泛素中水解氨基酸并切割双泛素。
患者/方法：纳入42例在小儿外科接受治疗的热水烫伤儿童，烧伤面积为4%-20%TBSA（年龄9个月至14岁，平均年龄2.5±1岁）。在受伤后2-6小时、12-16小时、3天、5天和7天，使用表面等离子体共振成像生物传感器评估血浆UCHL1浓度。18名因计划手术入院的健康受试者作为对照。
热损伤患者血浆中UCHL1浓度在热损伤后12-16小时达到峰值，随后随时间缓慢下降，在热损伤后第7天仍未恢复到正常范围。热损伤后UCHL1的平均浓度高于对照组测量范围（0.12ng/ml）：受伤后2-6小时-5.59ng/dl，受伤后12-16小时-9.16ng/dl，受伤后3天-6.94ng/dl，受伤后5天-5.41ng/dl，受伤后7天-4.09ng/dl。
我们观察到热损伤后2-16小时UCHL1浓度突然升高，随后随时间缓慢下降。UCHL1浓度与烧伤严重程度成正比。需要进一步研究以确定UCHL1在热损伤后代谢反应中的作用机制。
