Huang Xiaochen, Tang Keli, Xu Xuping, Cai Chunting
College of Life Science, Fujian Normal University, Fuzhou 350108, China; Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350001, China.
College of Life Science, Fujian Normal University, Fuzhou 350108, China.
Chemosphere. 2017 May;174:585-592. doi: 10.1016/j.chemosphere.2017.02.012. Epub 2017 Feb 4.
In this study, hydroponic experiments were conducted to investigate the role of Fe-Mn plaque and Arthrobacter echigonensis MN1405 under different solution Cd levels (0, 2 and 50 mg L) on Cd uptake and translocation by Phytolacca acinosa Roxb. (P. acinosa). Results showed Cd accumulated by P.acinosa without plaque were mostly distributed in root surface, indicating that an exclusion strategy for Cd tolerance. The formation of Fe-Mn plaque could contribute to the increase in the Cd tolerance of P.acinosa, promotion of the growth, increase in the enhancement of Cd translocation. Among all the treatments, Fe-Mn plaque treatments inoculated with MN1405 accumulated the maximum DCB-Cd (46.61 ± 6.36 g kg) and had the highest value of TF (2.14 ± 0.01) at 50 mg L and 2 mg L solution for Cd, respectively, demonstrating the greatest capacity to accumulate and translocate Cd. The uptake of Cd by P. acinosa in plaque treatments may result in the increase of soluble sugar and decrease of soluble protein synthesized from roots which involved in Cd detoxification and thus diminished the negative effects of Cd to some extent.
在本研究中，进行了水培实验，以探究在不同溶液镉水平（0、2和50 mg/L）下，铁锰氧化物胶膜和嗜碱节杆菌MN1405对商陆镉吸收和转运的作用。结果表明，无胶膜的商陆积累的镉大多分布在根表面，表明其对镉的耐受采用的是排斥策略。铁锰氧化物胶膜的形成有助于提高商陆对镉的耐受性，促进其生长，增加镉的转运。在所有处理中，接种MN1405的铁锰氧化物胶膜处理在镉浓度为50 mg/L和2 mg/L的溶液中，分别积累了最大量的二氯邻苯二酚提取态镉（46.61±6.36 μg/kg），且转运系数最高（2.14±0.01），表明其积累和转运镉的能力最强。在有胶膜处理中，商陆对镉的吸收可能导致根中合成的可溶性糖增加，参与镉解毒的可溶性蛋白减少，从而在一定程度上减轻了镉的负面影响。
