蓝莓饮料对成年大鼠慢性轻度应激所致认知障碍的保护作用。
Protective effects of blueberry drink on cognitive impairment induced by chronic mild stress in adult rats.
作者信息
Guo Qian, Kim Young-Nam, Lee Bog-Hieu
机构信息
Department of Food and Nutrition, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseon-si, Gyeonggi 17546, Korea.
Department of Food & Nutrition, Duksung Women's University, Seoul 01369, Korea.
出版信息
Nutr Res Pract. 2017 Feb;11(1):25-32. doi: 10.4162/nrp.2017.11.1.25. Epub 2016 Jun 30.
BACKGROUND/OBJECTIVES: Stress-induced cognitive impairment is related to the suppression of hippocampal neurogenesis that results from an increase of oxidative stress. Therefore, the aim of this study was to investigate the effects of administration of a blueberry drink, having a high antioxidant power, on the cognitive performance of adult rats exposed to chronic mild stress.
MATERIALS/METHODS: Twelve-week-old male Sprague-Dawley rats (n = 48) were randomly divided into four groups: control (CO), stress (ST), control + 5% blueberry drink (CO + B), and stress + 5% blueberry drink (ST + B). After eight weeks, the cognitive performance was assessed using a multiple T-maze water test. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and ascorbic acid were measured in the brain, and catecholamine concentrations were measured in plasma.
RESULTS
The brain weights of the rats from the ST and ST + B groups were significantly lower than those of the rats from the CO and CO + B groups. The cognitive performance of the ST group was impaired when compared to that of the CO group. This impairment was significantly improved by the blueberry drink supplementation ( < 0.05). The brain SOD and CAT concentrations were not influenced by the stress or by the blueberry drink. However, the brain levels of GPx and ascorbic acid were significantly lower in the ST group than those in the CO group and were increased by the blueberry drink supplementation. The plasma catecholamine concentrations were affected by chronic mild stress and by the blueberry drink. The plasma norepinephrine and dopamine concentrations were decreased by the chronic stress and improved by the blueberry drink supplementation. The plasma epinephrine level was only influenced by the stress.
CONCLUSION
These findings suggest that the blueberry drink may protect against the cognitive impairment induced by chronic mild stress.
背景/目的：应激诱导的认知障碍与氧化应激增加导致的海马神经发生抑制有关。因此，本研究旨在探讨饮用具有高抗氧化能力的蓝莓饮料对暴露于慢性轻度应激的成年大鼠认知能力的影响。
材料/方法：将12周龄雄性Sprague-Dawley大鼠（n = 48）随机分为四组：对照组（CO）、应激组（ST）、对照组 + 5%蓝莓饮料组（CO + B）和应激 + 5%蓝莓饮料组（ST + B）。八周后，使用多重T迷宫水试验评估认知能力。测量大脑中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和抗坏血酸的水平，并测量血浆中的儿茶酚胺浓度。
结果
ST组和ST + B组大鼠的脑重量显著低于CO组和CO + B组大鼠。与CO组相比，ST组的认知能力受损。补充蓝莓饮料后，这种损伤得到显著改善（<0.05）。大脑SOD和CAT浓度不受应激或蓝莓饮料的影响。然而，ST组大脑中GPx和抗坏血酸的水平显著低于CO组，补充蓝莓饮料后有所增加。血浆儿茶酚胺浓度受慢性轻度应激和蓝莓饮料的影响。慢性应激使血浆去甲肾上腺素和多巴胺浓度降低，补充蓝莓饮料后有所改善。血浆肾上腺素水平仅受应激影响。
结论
这些发现表明，蓝莓饮料可能预防慢性轻度应激诱导的认知障碍。
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