Louis Xavier Lieben, Thandapilly Sijo Joseph, Kalt Wilhelmina, Vinqvist-Tymchuk Melinda, Aloud Basma Milad, Raj Pema, Yu Liping, Le Hoa, Netticadan Thomas
Heart Failure Research Laboratory, Canadian Centre for Agri-Food Research in Health and Medicine, R2035, St. Boniface Research Centre, 351 Tache Avenue, Winnipeg, Manitoba, CanadaR2H 2A6.
Food Funct. 2014 Aug;5(8):1785-94. doi: 10.1039/c3fo60588d.
The purpose of this study was to examine the efficacy of an aqueous wild blueberry extract and five wild blueberry polyphenol fractions on an in vitro model of heart disease. Adult rat cardiomyocytes were pretreated with extract and fractions, and then exposed to norepinephrine (NE). Cardiomyocyte hypertrophy, cell death, oxidative stress, apoptosis and cardiomyocyte contractile function as well as the activities of calpain, superoxide dismutase (SOD) and catalase (CAT) were measured in cardiomyocytes treated with and without NE and blueberry fraction (BF). Four of five blueberry fractions prevented cell death and cardiomyocyte hypertrophy induced by NE. Total phenolic fraction was used for all further analysis. The NE-induced increase in oxidative stress, nuclear condensation, calpain activity and lowering of SOD and CAT activities were prevented upon pretreatment with BF. Reduced contractile function was also significantly improved with BF pretreatment. Blueberry polyphenols prevent NE-induced adult cardiomyocyte hypertrophy and cell death. The protective effects of BF may be in part attributed to a reduction in calpain activity and oxidative stress.
本研究的目的是检测野生蓝莓水提取物和五种野生蓝莓多酚组分对心脏病体外模型的疗效。成年大鼠心肌细胞先用提取物和组分进行预处理，然后暴露于去甲肾上腺素（NE）。在有或无NE和蓝莓组分（BF）处理的心肌细胞中，测量心肌细胞肥大、细胞死亡、氧化应激、凋亡和心肌细胞收缩功能，以及钙蛋白酶、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。五种蓝莓组分中的四种可预防NE诱导的细胞死亡和心肌细胞肥大。总酚类组分用于所有进一步分析。用BF预处理可预防NE诱导的氧化应激增加、核浓缩、钙蛋白酶活性增加以及SOD和CAT活性降低。BF预处理还显著改善了降低的收缩功能。蓝莓多酚可预防NE诱导的成年心肌细胞肥大和细胞死亡。BF的保护作用可能部分归因于钙蛋白酶活性和氧化应激的降低。
